Through this page, we want to explain the role that each team member has had within the project. We want also to make known, and thank, the other actors of the project who participated in its smooth running.
But before that we will present rapidly our iGEM Grenoble history!
iGEM GRENOBLE-ALPES STORY
1) How did we know iGEM ?
We have never had lessons on iGEM but each member of the team has heard about the competition at Grenoble Alpes University (UGA) or INP school, by the members of the previous team and the teachers who took them framed.
Then, the team met last November via Pierre Cavailles (the main advisor and a biotechnology teacher at the UGA), and that's how the story started.
2) Project Chronology
You can find, in the diagram below, the chronology of the project which summarizes the key points of our work.
NEED DIAGRAM
Attribution
This part describes the work done, and the person who performed it.
The legend below could help you to know if it is a team member, an advisor, an instructor or an outsider thanks to the color code :
Team members Advisors Instructors Outsiders
Engineering
DNA extraction module & Phages-bacteria Modeling Thomas Goyet
Temperature control system & Transformation Module Xandre Gallard
Fluorescence Sensor module Charles Jabour
Pipetting Module Jean Le Pennec
Overall 3D Model of the system
Mainly Lucas Sancéré with the help of Jean Le Pennec & Thomas Goyet
Overall code for the control of the system Jean Le Pennec & Thomas Goyet
PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:
Lab and premise support
Ugo Hevin, Franz Bruckert & Marianne Weidenhaupt of the CIME Nanotech Laboratory (Minatec, Grenoble)
Nicolas Ruty & Julien Traveaux of the Phelma-INP school
Difficult technique support
Engineers of BioMérieux : Hervé Rostaing (general engineering support), Blaze Jérome (mechanics and machining of complex parts of the system), Frédéric Pinston (fluorescence detection) & Alain Laurent (magnetic beads).
Biology
Experiments on bacteriophages
& their capacity to lyse bacteria Cécile Razimbaud & Yasmine Taveau
DNA extraction with magnetic beads Cécile Razimbaud
Construction of the DNA probe :
- detecting an antibiotic resistance marker of Pseudomonas a. PA01 Elise Aubert
- detecting a sequence of an housekeeping gene of Pseudomonas a. PA01 Fanny Garon
Conservation of biological products & Freeze-drying Perrine Rochas
BBa_J04450 characterisation Perrine Rochas with the help of Xandre Gallard
PEOPLE WE WANT TO THANK FOR THE HELP PROVIDED TO THE TEAM IN THIS WORK:
Lab support
Sophie Boretti, Martin Ghis and “practical work” service members in general (first floor of Jean Roger Laboratory (University of Grenoble-Alpes)).
The fifth floor members of Jean Roger Laboratory including Pierre Cavailles. Hélène Coradin, Béatrice Schaack (for her freeze-drying help), and the other members of the eighth floor of Jean Roger Laboratory.
Biology support
Pierre Cavailles & Clément Caffaratti who provided a lot of advice about the Biology and an outstanding support throughout the project.
Bacteriophages experiments support - General support & donation of T5 bacteriophages and their host E. coli F
Cécile Breyton of the Grenoble IBS (Institute of Structural Biology)
Donation of Pseudomonas a. PA01
Ina Attrée of the Grenoble CEA (Office of the Atomic Energy and Alternative Energies)
Donation and sending of PAK-P3 bacteriophages and their host Pseudomonas a. PAK
Laurent Debarbieux of the Pasteur Institute of Paris
Donation of Staphylococcus aureus strains and a specific bacteriophage Sb-1
Grégory Resch & Jonathan Save of the University of Lausanne (Swiss)
Feedback and advices about detection experiments on bacteriophages
Pierre Marcoux of the Grenoble CEA (Office of the Atomic Energy and Alternative Energies)
FURTHER INFORMATIONS AND ACKNOWLEDGEMENTS
Pseudomonas aeruginosa PAO1 and their specific phages HER18 the team needed to find a class 2 laboratory which accepts to welcome us. Javier Yugueros Marcos, the main BioMérieux contact, have immediately responded to this call for help. With the assistance of Patrice Gracieux, he organized the coming of Cécile Razimbaud in BioMérieux lab (about security and others). Stéphanie Borland has accompanied Cécile during all the experiments, mostly for the safety rules.
