Team:Calgary/Protocols

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PROTOCOLS

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Below are the protocols used by the team.

REHYDRATION OF REGISTRY DNA

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

PROTOCOL 2

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

PROTOCOL 3

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

PROTOCOL 4

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

PROTOCOL 5

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

PROTOCOL 6

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.