Team:Madrid-OLM/Contribution

• Team
  • Members
  • Attribution
  • Sponsors
• Project
  • Description
  • Contribution
  • Improve
  • Demonstrate
  • Judging Form
• Aptamers
  • Overview
  • Discovery
  • Characterization
  • Computational
    Improvement
  • Binding to Electrode
• Device
  • Overview
  • First Prototype
  • Final Prototype
    • Overview
    • Microfluidics
    • Electronics
• Experiments
  • Interlab
  • Protocols
    • Aptamer Related
    • Manufacturing
• Human Practices
  • Integrated Human Practice
  • Education and
    Engagements
  • Collaborations
• Awards
  • Hardware
  • Applied Design
  • Entrepreneurship
  • Measurement
• Medals

Contribution

During this competition, we have faced a different kind of problems, but the principal ones were the lack of money and time. We have felt the absence of resources in both of the parts of our project, the aptamer development, of the device manufacturing. For solving this issue we have learned to make all the equipment that we had need during the project by ourselves, getting the inspiration in the DIYBio movement. Now we are proud to contribute to the iGEM community with a new successful way of:

1. Making affordable PDMS chips with a new manufacturing workflow.

Microfluidics

2. Helping future teams to discover more and more aptamers through a new SELEX protocol based on 3D printed nitrocellulose columns.

Selex Protocol

3. Developing a new quantitative method for aptamer characterization, improving the earlier semi-quantitative ways.

Aptamer Characterization

4. Establishing a simple and cost-effective method to make electrochemical measurements of protein concentration with aptamers.

Binding to electrode

5. Integrating all the technologies in our final design, which is an automated device for aptasensing, easily replicable as long as it is made with digital fabrication techniques.

Final Prototype