Team:Calgary/Notebook - 2018.igem.org

PROTOCOLS

Below are the protocols used by the team.

Experimental Details	This section might be deleted.
Materials	iGEM 2018 distribution kit ddH₂O
Protocol	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA as per transformation protocol. Store the remaining amount at -20°C.

Experimental Details	This section might be deleted.
Materials	Synthesized DNA from IDT or Genscript ddH₂O
Protocol NEED TO VERIFY	Centrifuge tube containing the synthesized DNA for 3-5 seconds at 3000g to ensure that all material is at the bottom of the tube. Add ddH₂O to reach a final concentration of 50 ng/μL. Vortex. Incubate at 50°C for 20 minutes. Briefly vortex and centrifuge. Store at -20°C.

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

Experimental Details	Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.
Materials	iGEM 2017 distribution kit ddH₂O
Protocols	Add 10μL of ddH₂O to the desired well. Pipette up and down 3-5 times. Incubate at room temperature for 10 minutes. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

Team:Calgary/Protocols