Team:Calgary/Description

Team:Calgary - 2018.igem.org/Description

OUR PROJECT


Integration

Developing a cell line capable of simple, targeted, and large gene insertions

The "Integration" stage of our project was designed to develop a cell line which could facilitate the simple insertion of recombinant DNA into pre-made "socket" within the genome. This was attempted through two main steps: A CRISPR/Cas9 step and a recombinase-mediated step.

UCOE

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CRISPR

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UCOE

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Software/Modelling

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