Team:Calgary/Description

Team:Calgary - 2018.igem.org/Description

OUR PROJECT



Integration

Developing a cell line capable of simple, targeted, and large gene insertions

The Integration stage of our project was designed to develop a cell line which could facilitate the simple insertion of recombinant DNA into pre-made "socket" within the genome. This was attempted through two main steps: A CRISPR/Cas9 step and a recombinase-mediated step.

FLP-Beta

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CME

Developing a cell line capable of simple, targeted, and large gene insertions

The Integration stage of our project was designed to develop a cell line which could facilitate the simple insertion of recombinant DNA into pre-made "socket" within the genome. This was attempted through two main steps: A CRISPR/Cas9 step and a recombinase-mediated step.


Microfluidics

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Software

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