Team:Madrid-OLM/Demonstrate

• Team
  • Members
  • Attribution
  • Sponsors
• Project
  • Description
  • Contribution
  • Improve
  • Demonstrate
  • Judging Form
• Aptamers
  • Overview
  • Discovery
  • Characterization
  • Computational
    Improvement
  • Binding to Electrode
• Device
  • Overview
  • First Prototype
  • Final Prototype
    • Overview
    • Microfluidics
    • Electronics
• Experiments
  • Interlab
  • Protocols
    • Aptamer Related
    • Manufacturing
• Human Practices
  • Integrated Human Practice
  • Education and
    Engagements
  • Collaborations
• Awards
  • Hardware
  • Applied Design
  • Entrepreneurship
  • Measurement
• Medals

Demostrate

Our initial idea has always been to implement a feasible way of getting to what we have called “the Internet of BioThings”.

LINK A OUR VISION. IoBT

To get there, we had to test four different and correlated sections:

1. Being able to obtain accurate protein concentration data from the environment.

2. Automating the protocol of analysis for ease of measurement replication and calibration.

3. Uploading the data obtained to the cloud in real time.

4. Designing a scalable process looking forward to integrating eventually new target molecules.

Microfluidics

1. Helping future teams to discover more and more aptamers through a new SELEX protocol based on 3D printed nitrocellulose columns.

Selex Protocol

2. Developing a new quantitative method for aptamer characterization, improving the earlier semi-quantitative ways.

Aptamer Characterization

3. Establishing a simple and cost-effective method to make electrochemical measurements of protein concentration with aptamers.

Binding to electrode

4. Integrating all the technologies in our final design, which is an automated device for aptasensing, easily replicable as long as it is made with digital fabrication techniques.

Final Prototype