Team:Pasteur Paris/Demonstrate
NERVE GROWTH FACTOR AND NEURON CULTURE
We successfully cloned a part coding for secretion of NGF in pET43.1a and iGEM plasmid backbone pSB1C3, creating a new part Bba_K2616000 and confirmed the production of proNGF by Western Blot and mass spectrometry.
We grew neurons on our self-made microfluidic chips ans successfully observe axon growth in the presence of commercial NGF
KILL SWITCH
Achievements:
- Successfully cloned a part coding for toxin/antitoxin (CcdB/CcdA) system in iGEM plasmid backbone, creating a new basic part Bba_K2616002
- Successfully sequenced BBa_K2616002 in pSB1C3 and sent to iGEM registry
- Successfully observe survival of our engineered bacteria at 25°C and 37°C and absence of growth at 18°C and 20°C, showing the efficiency of the kill switch
Next steps:
- Find a system that kills bacteria when released in the environment rather than just stopping their growth