Team:Calgary/Description
OUR PROJECT
CRISPR
Developing a cell line capable of simple, targeted, and large gene insertions
The Integration stage of our project was designed to develop a cell line which could facilitate the simple insertion of recombinant DNA into pre-made "socket" within the genome. This was attempted through two main steps: A CRISPR/Cas9 step and a recombinase-mediated step.
FLP-Beta
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CME
Developing a cell line capable of simple, targeted, and large gene insertions
Gene inserts are at risk of being rendered ineffective even after successful integration into the genome, as the spread of heterochromatin and DNA methylation can cause gene silencing. Furthermore, regulatory elements within both the insert and genome near the locus of integration may interact bidirectionally, leading to changes in gene expression known as neighbourhood effects. Chromatin modifying elements (CMEs) can help to generate an isolated, protected pocket within the genome, thereby assuring stable and sustained expression of integrated genes within eukaryotic systems.
Microfluidics
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Software
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