Why didn’t the colonings work? Possible reasons for our failure are that we tried to perform a PCR using the wrong primers or that we committed an error somewhere else in the procedure. Another possibility is that we had the wrong stem of Bacillus subtilis from the beginning, because only the stem 168 has the required genes.
We also tried to order the parts for our clonings from IDT, but they were not able to send them to us in time for us to still work with them.