Team:Jilin China
ABSTRACT
Manystrategies could be used by bacteria to coordinate temperature-dependent gene expression. A well-known class of biological temperature sensitive element is RNA-based thermosensor (RT), which is thermoregulatory RNA sequence in the 5'-untranslated region of mRNAs. RNA thermosensors could induce equilibrium shift between closed and open conformations of the translation initiation region under temperature variation, which influences the mRNA degradation or ribosome accessibility, thereby controlling translation efficiency.
However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation on their potential application in many areas. To address this issue, based on free-energy method, we created synthetic RNA-based thermosensors (SynRTs) toolkit, which have broader sensing range. Futhermore, we built a matched search engine for potential users in numerous foundational and applied areas,
The SynRT toolkit have updated from version 1.0 to 3.0, which is integrated with our human practices. The newest version is composed of four types of SynRTs:
Hot-induced 5'UTR
Human Practices
Model
More
Title