Team:Jilin China
ABSTRACT
Many strategies could be used by bacteria to coordinate temperature-dependent gene expression. A well-known class of biological thermoregulatory element is RNA-based thermosensor (RT), which is located in the 5'-untranslated region of mRNA. RTs could sense temperature change and influence the mRNA degradation or ribosome accessibility, thereby controlling translation efficiency.
However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation on their prospective application. To address this issue, we created synthetic RNA-based thermosensors (SynRTs) toolkit responding to different sensing temperatures and different intensits. Futhermore, we built a matched search engine for potential users.
The SynRT toolkit have updated from version 1.0 to 3.0, and the newest version is composed of four types of SynRTs:
Hot-induced 5'UTR
Human Practices
Model
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