Attributions

We express our deepest gratitude to all the people who have made this project possible. Both those who have helped us through funding, and all those who have helped us with their advice, giving us materials or reagents and protocols. This includes all the teachers, researchers and colleagues who have supported and advised us. Also we express our gratitude to the universities, companies and public entities that have supported us through the cessation of laboratories, places to organize congresses and conferences held throughout the process. The trust that has been placed in us has been immense, despite being just students. We know that you are busy people, and still you have taken time to attend us and clear a lot of doubts. That is something not everyone does, and for that we want to reiterate our deepest gratitude. If something big comes out from this project, it will be because of your help.

Members

Francisco Javier Quero Lombardero has been working both in the wet lab and the manufacturing section. In the laboratory, he has been working in the aptamer discovery, characterization and integration sections. Related to aptamer discovery, he has designed and manufacture the nitrocellulose columns and its implementation. He has also designed the qPCR experiments for SELEX round checking. In the aptamer characterization, he has been working trying the different aptamer characterization techniques as well as developing the final one, the ELONA. He has also carried out the experiments to integrate the aptamer into the electrode. In the hardware section, he has been working designing the PDMS chips and making the manufacturing process (Hacking the laser cutter, developing plasma generation, helping with vacuum chamber fabrication…). He has also designed, assembled and tested all the electronics. Regarding the software, he has written the code of each control component of the system (Arduino, Rodeostat, and PC). He has also talked with sponsors to raise funds.
Laura Armero Hernández has been working in the aptamer discovery section by performing and perfected the SELEX process, for both proteins, and designing the qPCR experiments for SELEX round checking. She has also helped in the last aptamer characterization technique, the ELONA. She has been dealing with administrative chores of the team and helped to look for funding.

Rodrigo Hernandez Cifuentes has been working in the hardware section, developing the electromechanical systems for the device: microfluidics chips, pressure pumps, a design-oriented to manufacturing and the overall integration of the subsystems into a piece of hardware. From the experimental side, he has been involved in hacking the microwave, the laser cutter, the compressor-pump transformation, setting up of the vacuum chamber, generating plasma, assembling the designs. He has also helped with the electrochemical measurement.

Iván Martín Hernández has been working in the elaboration, management and developing the wiki. In the aptamer section, he was in charge of the computational improvement of the aptamers. He also had been doing the collaborations with Tec-Monterrey team. He also helped in the laboratory when it was needed. He represented the team in the "Mediterranean" meet up and in the "Spanish" meet up. He also helped in the organization of "Another European" meet up.

Borja Sánchez Clemente has been working in the design and signal processing of the optical prototype. He has also helped with the electrochemical measurement.
Ignacio Albert Smet has been working in the design and signal processing of the optical prototype. He has also helped with the Human Practices and organizing the Spanish meetup.

Guillermo Fernández Rodríguez has been working with Interlab and in the aptamer characterization section (ELONA).
Gonzalo Saiz Gonzalo has been working in the Interlab protocol. He has also helped to look for sponsors. He represented the team in the "Mediterranean" meet up and helped the iGEM guide for new teams in Spain.
Mentors

Domingo Marquina Díaz was the principal PI of the team. He was the first person to believe in the project, helped us developing it, encouraged and supervised us during all the journey.

Rafael Alejandro Alonso Conde has helped, as our secondary PI, a lot in different aspects whenever we need help: management, purchase of materials, manufacture of acrylamide gels and help in the realization of the Elona protocol.

Maria Teresa Lopez Jaen contributed to developing the initial idea of the project and generously lend us her lab space and equipment to conduct our experiment. She was always free to give us advice or a hand.

Cristina Sanchez Garcia has spent all her efforts in obtaining the university support funds for our team and the materials we have been needing during the year, help to deal with the administration and help us whenever we have needed. She has also promoted the iGEM initiative inside the university and helped us organized all the events related to this matter.

Maria Teresa Villaba Diaz has kindly donated all the protein needed to carry out all the experiments and answer all our doubts. She was also in touch and wanted to stay updated with our progress, cheering us.

Victor Manuel González Muñoz and Maria Elena Martín Palma: have guided the team during all the SELEX and aptamer characterization experiments ( even sharing their protocols) and ultimately lend us their own space in the Ramon y Cajal Institute to make some experiment there.

Jose Ignacio Klett selflessly spend two days of his own working schedule to help the team in the realization of the experiments in the Ramon y Cajal Institute.

Susana Campuzano Ruizhas advised about the aptamer measurement, helping us to understand the technique and advising about the material and equipment best to perform it.

Victor de Lorenzo has always a good disposition to talk with us and help in the plasmid binding and cloning of biobricks, and making time to give a lecture during the meetup we hosted.

Tomas Aparicio has helped the team to sketch all the plasmid binding and cloning of the biobricks, during his work hours.
Esther Lantero Bringas has taught us how to clone (the Interlab section would not have been possible without her) and she has supported us all year long.
Helping hands

Marcos Hernandez Cifuentes has helped developing an app, during his free time.

Elena Benito Peña kindly lend us some PDMS, when we run out of it, to continue with our experiments.
Maria del Carmen Moreno Ortiz (laboratory technician) has been helping us all year and become essential to our survival in the department.
Pilar Arana always showed interest in our progress and help us with her vastly experience in DNA amplification.

Yasmina generously gave us a space in her department to mount there our hardware lab until the end of the competition, and checked on us regularly to see if we needed something.

David Alfaro Sanchez has always been willing to lend the team basic lab material.

Luis Fernández Pacios has provided information and knowledge about protein structure prediction.

Emilia Lopez Solanilla has provided information and knowledge about cloning protocols.

Saul Ares and Krzysztof Wabnik took the time to prepare both prepare a lecture for the meetup we hosted.

Pablo Quesada was generous enough to cut all the methacrylate structure for our device.

Marta Montero illustrated and designed the model for our banner..

Jose Antonio Hernández and Felisa Cifuentes provided the needles and syringes of several ranges to proved differents experiments when developing the microfluidics, as well the ones used in the final prototype.
Javier Gonzalez and Alvaro Ramos are teachers of TEMS School and helped us to organize the presentation that we did in this school.

Team:Madrid-OLM/Attributions

Attributions