Team:NEFU China/Splicing

Splicing


Splicing

Transformation of information
Every letter has its own frequency in English words, so does each codon in organisms. Therefore, we created a coding list, in which each letter corresponds to several codons with the same frequencies. In addition, we composed a program that can easily convert English sentences into DNA sequences.
We have made the transition from nucleotide sequences to DNA barcode.
Information expression verification
Yeast: a-type yeast.
Function: Using the specific “key-lock” interaction, information can be obtained when expressing a specific key.
Vector construction:
Gene fragments of information with a stem loop and URA screening genes were ration inserted between the two homologous arms, HA1 and HA2 to construct the pesc-ura-stem loop-information-URA plasmid. HA1 and HA2 act as homology arms to promote the homologous recombination, which allows the integration of the DNA containing the stem loop lock sequence into the yeast genome. (配图)
Functional verification:
Plasmids with information fragments were transformed into yeast. Then we extracted the total RNA of yeast and carry out reverse transcription. DNA sequencing will be used to verify the correct expression of the information fragment is expressed, which will be compared with the original information.