Team:Rheda Bielefeld/Cloning Strategy

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Cloning Strategy

Cloning is one of the most important points at the end. We had cloned Bacillus subtilis and Xanthomonas campestris to isolate their DNA in large amounts.
Afterwards we wanted to pull out the Gene of Interest (GOI).
Those were pelB and yesZ of both of the bacteria.
Then we would have built the vector which is used for every plasmide with pz9 for the characterization and psB1C3 for our BioBrick. Next we would have put our Gene of Interest into the vector and duplicate it via PCR. At last we would have need to place our finished plasmide in our E. coli and would have cloned our final bacterium.