Protocol
1. PCR system
1) 20μL system is used for verification
Taq:
Super mix:
2) 50μL system for PCR target genes
Pfu mix:
DNA: plasmid 50ng; genome 100~200ng
3) 20μL PCR system for microbes
Super mix:
Bacteria (after picking on the board, back up on the new board and rinse it in the system that has been prepared)
