Team:Pasteur Paris/Acknowledgements
Thank you to all the professionals who advised us
Microbiology
Finn Stirling, Pamela Silver Laboratory, Harvard Medical School, Boston MA 02115 For letting us use the cryodeath kill-switch he invented. He gave us the sequence of the gene which we used as a base to develop our own design and shared a lot of advice, suggestions, and tips for our project.
Dr. Guillaume Dumesnil, Unité Pathogène des Infections Vasculaires, Institut Pasteur, 75015 Paris For allowing us to have access to his laboratory to realize our electrophoresis experiments.
Pr. Jean-Marc Ghigo, Unité Génétique des biofilms, Institut Pasteur, 75015 Paris For sharing his expertise about biofilms, discussions, protocols for biofilm culture, experimental design and for supplying a strain of GFP-fluorescent Staphylococcus aureus.
Nathalie Béchon, Unité Génétique des Biofilms, Institut Pasteur, 75015 Paris For giving us some advice about our biofilm protocols, discussions and for providing us with a solution that enabled to fix our biofilms in the 96-well plates.
Dr. Nolwenn Jouvenet, Team Génomique Virale et Vaccination, Institut Pasteur, 75015 Paris For lending us their 96-well plate reader.
Takara Clontech, 78100 Saint-Germain-en-Laye For providing us with an In-fusion kit, and by giving us insightful advice on how to use their kit.
Dr. Victor de Lorenzo, Molecular Environmental Microbiology Laboratory, Centro Nacional de Biotecnología, 28049 Madrid, For sending us a stock of bacteria transformed with a plasmid we wanted to use: pVDL 9.3.
Cellular Biology
Dr. Amélie Wegener, Team Présentation de l’antigène par les cellules dendritiques, INSERM U1016 - CNRS UMR8104 - Université Paris Descartes, Institut Cochin, 75014 Paris For sharing with us her protocols for myelinating oligodendrocytes-DRG co-cultures. She also gave us some advice about the experiments we could perform.
Dr. Bernard Zalc, Team Développement oligodendrocytaire et interactions neurovasculaires, Institut du Cerveau et de la Moelle épinière (ICM), 75013 Paris For giving us a better understanding of nerve growth and some tips regarding their growth. He also explained to us the connection between the nerve and our interface.
Dr. Chiara Zurzolo, Unité Trafic membranaire et Pathogenèse, Institut Pasteur, 75015 Paris For letting us to manipulate our neuronal cells in her L2 lab.
Dr. Christine Rasetti-Escargueil, Unité des Toxines Bactériennes, Institut Pasteur, 75015 Paris For giving us important advice about the coating of our microfluidic chips.
Dr. Emmanuel Lemichez and Dr. Amel Mettouchi, Unité des Toxines Bactériennes, Institut Pasteur, 75015 Paris For letting us use their L2 lab to manipulate our neuronal cells.
Dr. Frida Loria Salinas, Unité Trafic Membranaire et Pathogenèse, Institut Pasteur, 75015 Paris For letting us manipulate in her lab, and for sharing with us some of her protocols about cell dissociation from fresh tissues.
Dr. Han Li, Team Plasticité Cellulaire et Modélisation des Maladies, CNRS UMR 3738, Institut Pasteur, 75015 Paris For giving us access to the L2 laboratory.
Dr. Pauline Spéder, Team Plasticité Cérébrale en réponse à l’environnement, CNRS UMR 3738, Institut Pasteur, 75015 Paris For letting us proceed with some protocols for the neuronal culture in her lab.
Stéphanie Chrupalla, Thermo Fisher Scientific - Life Science Solutions, 91963 Courtaboeuf For lending us an iBlot2 for the protein transfer from a gel to a membrane.
Microfluidics
Dr. Ayako Yamada, CNRS, UMR8640 Processus d’Activation Sélectif par Transfert d’Energie Uni-électronique ou Radiatif, ENS-Département de chimie, Paris For providing us the right contacts.
Dr. Catherine Villard, Unité de Physico-chimie, UMR168, Institut Curie, 75005 Paris For advising us on microfluidic chips and for letting us their own design and lab.
Dr. Frédéric Kanoufi, ITODYS, CNRS-Université Paris Diderot, Paris For providing us a lot of advice about electrodes.
Gregory Rousselot, Sterlitech Corporation, WA 98032-1911 United States For providing us with (free) gold-coated polycarbonate and alumina oxide membrane filters, that were crucial for our project.
Dr. Heng Lu, Laboratoire de Biochimie (LBC), ESPCI-CNRS, Paris For advising us on microfluidics, helping us to find the right contacts, teaching us AutoCAD and providing us with ideas.
Pierre Roussel-Ragot, UMR7587 Institut Langevin Ondes et Images, Institut Langevin, 75005 Paris For giving us advice on the electronic part of our project and for giving us electronic equipment.
Dr. Yacine Bounab, Microbiology Technology Institute - BIOASTER Paris, 75015 Paris For giving us a lot of advice on our project and for letting us use their safety cabinets.
Dr. Yann Verdier, Team Spectrométrie de Masse Biologique et Protéomique, ESPCI, 75005 Paris For giving us advices, materials and the access to the lab.
Modeling
Dr. Anđela Davidović, Institut Pasteur, Expertise group : SABER – Statistics applied to biology and experimental results For helping us to start the model and giving us tips about our model.
Dr. Jean-Marc Victor, Laboratoire de Physique Théorique de la Matière Condensée (LPTMC), CNRS UMR7600, Sciences Sorbonne Université, 75005 Paris For helping us to understand some articles about the modeling of NGF.
Design
Albane Imbert, FabLab, Institut Pasteur, 75015 Paris For giving us access to the Institut Pasteur FabLab and for teaching us how to use the machines for prototyping (3D printer).
Dr. Eric Denes, Dr. Evelyn Poli, Dr. Christelle Arico, I.CERAM, 87068 Limoges For inviting us to visit their factory, for giving us some metallic pieces (titanium TA6V4, stainless steel 316L) to assess the biofilm formation. They allowed us to have a better understanding of the industrial and medical constraints of the implantations of inorganic materials inside the human body.
Communication
Alban Orsini, Sabine D'Andrea and Jean-François Chambon, Institut Pasteur, 75015 Paris For teaching us the rules of online communication and giving us some advice about what and when to post.
Financing
Pr. Emmanuel Fort, ESPCI, 75005 Paris For financing some important equipment, like conductive silver paste and rat cortex, and taking care of the logistic matter.
Nathalie Bousseau, Fondation Centrale-Supélec, 91190 Gif-sur-Yvette For financing a part of our project.
Dr. Pierre Ougen, Team S.B.I.P., Sup’Biotech Paris, 94800 Villejuif For financing all the electric components we used and always being present to give us advice when we needed it.
Dr. Vanessa Proux, Sup’Biotech Paris, 94800 Villejuif For financing a part of our project and some of 4 of our plane tickets to Boston.
Dr. Véronique Bellosta, ESPCI, 75005 Paris For supporting our team with financial help and promoting iGEM at ESPCI Paris.
Legal
Delphine Tarrete, Institut Pasteur, 75015 Paris For taking care of all our internships agreement and the NDA.
Management
Chantal Sobczyk-Moran and Natacha Girold, Institut Pasteur, 75015 Paris France For taking care of business administration and account management.
Other
Pr. Antoine Latreille, Doyen de la Faculté Jean Monnet, 54 Boulevard Desgranges, 92330 Sceaux For allowing us to organize the Parisian Meet-up in his faculty.
Dr. Alexandre Ismael, Collaborative Research Project, Sup'Biotech, 94800 Villejuif For sharing with us his expertise about the iGEM competition and for following our work.