togglepH
VIT Vellore
We use a plasmid system consisting of a composite part with two promoters
This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH
Repressor molecule LexA is produced in order to repress the expression of the biobrick at low pH
LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, a recA protein is produced, which binds to the repressor and prevents its action, thereby regulating the process