ATTRIBUTIONSAttributions
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What are we doing?
We construct our promoter library with the free combination of core promoter and 5'UTR, then the expression efficiency is easily to be changed with the replacement of different core promoters and sequence changes of 5’UTR, realizing the bi-level regulation of both transcription and translation. Four synthetic standard regulated libraries have been constructed:
- (1) Heat-induced RNA thermosensors library, directional reconstructions to secondary structures of RNA thermometers;
- (2) Heat-repressible RNA thermosensors library, directional reconstructions to anti-RNaseE cleavage site;
- (3) Cold-induced RNA thermosensors library, directional reconstructions to mRNA pseudoknot of CspA;
- (4) Cold-repressible RNA thermosensors library, directional reconstructions to RNase III binding site.