Parts Overview
We relied heavily on three basic parts from the Registry (CMV,GAL4 and VP16), and so we changed several sites to optimize them. We designed a new composite part BBa_K2580666 to express GAL4-VP16, an potent transcriptional activator[1] , to drive other parts’ expression.
[1] Sadowski I, Ma J, Triezenberg S, et al. GAL4-VP16 is an unusually potent transcriptional activator[J]. Nature, 1988, 335(6190): 563.