Team:Goettingen/Part Collection

Contact:

E-mail:	igem2018@gwdg.de
Adress:	c/o iGEM 2018
	Department of General Microbiology
	Grisebachstraße 8
	37077 Göttingen
	Germany

We are especially grateful to our numerous sponsors on gofundme, who supported our project and made it possible.
Every public donor, who helped us to achieve our goals got a special place on our bacterial wall of fame and a handdrawn microbe to show our appreciation. A link to that wall can be found here:

Bacterial Wall of Fame

Part collection for glyphosate resistance

In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil bacterium B. subtilis is glyphosate resistant. The target of glyphosate is the EPSP synthase, which is encoded by the aroE gene in B. subtilis and by the aroA gene in E. coli. If the target is mutated, like in our part BBa_K2586020, the cells are characterized by a high glyphosate resistance. If the cells express the gene encoding the glyphosate-N-acetyl-transferase (GAT), they are also resistant. In conclusion, our collection can serve as a powerful toolbox to genetically engineer organisms for enhancing the resistance towards glyphosate and to improve inactivation or degradation of the weedkiller by microbes.

Part Number and Name	Short Description	Type	Length [bp]
BBa_K2586001/gltT	Uptake of glutamate or glyphosate from the environment	Coding	1290
BBa_K2586002/gltP	Uptake of glutamate or glyphosate from the environment	Coding	1245
BBa_K2586003/aroE	Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate	Coding	1287
BBa_K2586007/aroA	Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate	Coding	1290
BBa_K2586019/gat	Glyphosate N-acetyl-transferase	Coding	480
BBa_K2586020/aroA*	Mutated aroA	Coding	480