Team:SKLMT-China/Basic Part

Basic Part

Standard promoter biobrick

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Overview

Our submitted parts include a promoter library of inner promoters with various strength built in Pseudomonas fluorescencepf-5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhanced nicotine degradation compared to original nicA2 gene.

Promoter Library

We are proud to submit a promoter library of inner promoters with various strength built inP.fluorescencepf-5. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in P.fluorescencepf-5. Additionally, this promoter library will support future iGEM teams some help in exploration on P.fluorescencepf-5 cells with high capacity in degrading.

We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.

According to friend teams’ collaboration datas and our analysing, we built a model to regularize and predict the strength of library members. You can learn about these members intuitively.

All Basic Parts

Biobrick	Type	Description	length
BBa_K2569001	Regulatory	Pdiv, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569002	Regulatory	ParaA, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569003	Regulatory	PfabD, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569005	Regulatory	PccmD, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569006	Regulatory	PfliA, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569011	Regulatory	PpltR, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569012	Regulatory	PproC, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569013	Regulatory	PrecA, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569014	Regulatory	PrpoD, a constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569015	Regulatory	PrpoS, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569016	Regulatory	PdnaA, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569017	Regulatory	Ppol, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569018	Regulatory	P16s, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569019	Regulatory	Psig, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569020	Regulatory	Patf, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569021	Regulatory	Pfme, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569022	Regulatory	Pmem, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569023	Regulatory	Pfat, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569024	Regulatory	Pami , constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569025	Regulatory	Prib, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569026	Regulatory	Pabc , constitutive promoter in pseudomonas fluorescence pf-4	70
BBa_K2569027	Regulatory	Pedo , constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569028	Regulatory	Ptox , constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569029	Regulatory	PampC , constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569030	Regulatory	Plip , constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569033	Regulatory	PampR,promoter	92
BBa_K2569034	Regulatory	Pdiv, constitutive promoter in pseudomonas fluorescence pf-5	70
BBa_K2569036	Coding	Nicotine oxidase NicA2	1449

 

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