This year, the iGEM Team TecMonterrey_GDL decided to contribute to the Interlab study measurements. The main goal of the study was to achieve a way to reduce lab-to-lab variability in fluorescence measurements through the normalization of absolute cell count or colony forming units.
The approaches taken were indicated by the iGEM measurement team:
- 1. Converting between absorbance of cells to absorbance of a known concentration of microsphere silica beads
- 2. Counting colony-forming units (CFU) from the samples
The team followed a series of protocols created to help standardize the measurement of GFP (green fluorescent protein), which is widely used as a measurement marker.