Team:TecMonterrey GDL/Protocols

Protocols

For E.coli :

• View Preparation of LB Medium
• View Transformation by electroporation
• View Calcio competent cells
• View Ligation and digestion of G-Blocks
• View Transformation Heat Shock

• View LB medium and LB agar-plates Protocol
• View MINIPREP KIT
• View PCR
• View Agarose Gel Electrophoresis
• View Optimizing Restriction Endonuclease Reactions

• View Competent cells
• View Protein extraction and purification based on Thermo Fisher's Protocol
• View SDS-PAGE
• View Western Blot based on Invitrogen's protocol

For Peptides :

• View Competent cells
• View Procedure of digestion of the constructo
• View PCR

• View Transformation by electroporation
• View High Fidelity restriction enzyme Protocol
• View MINIPREP KIT

• View Calcio competent cells
• View Protein Expression with T7 Strains

For L. rhamnosus GG :

• Electrocompetent cells protocol optimized for L. rhamnosus GG
• View Plasmid isolation for Lacatobacillus spp.

• View Electroporation of L. rhamnosus GG.
• View Total DNA extraction L. rhamnosus GG

TecMonterrey_GDL