Internal protocol for the experiments performed in the confocal microscope with supported lipid bilayers. The protocol contains parts from other external protocols.
Protocol for experiments with protoplasts. We have based our protocol losely on the Hokkaido 2011 iGEM teams protocol. We used the same buffers but prepared the protoplasts differently.
This protocol describes our strategy when assembling of the constructs that we worked with in lab.
This protocol was provided to us from our supervisors in lab.
Protocol overview describing the different experiments we performed with SIEC secretion into liposomes.
Protocols from other sources:
Protocol from iGEM HQs. We use LB media instead of SOC media.
We used this iGEM protocol whenever making our competent cells. LB media was used instead of SOC media. In step 14, we aliquot 50 uL into 1,5 mL eppendorf tubes. (Nat should proofread this!).
Protocol from Nikos Hatzakis’ enzyme lab. Explains the process of making liposomes. When making liposomes in our own lab, we used liquid nitrogen instead of acetone and dry ice.
Excel sheet to calculate amount of lipid added when making liposomes. Goes with the protocol Liposomes preparation step-by-step.
When making supported lipid bilayers it is based on this protocol step 8 to 12.