All student team members performed outstanding work and showed interest and dedication to the project. As we are a rather small team, we did not have a strict division of roles and many times we worked all together to accomplish certain parts of our project.
Our project was inspired by the works of iGEM Peking 2015 and iGEM UiOslo_Norway 2016. Our decision to detect vulvovaginal Candida albicans stemmed from the public lack of knowledge concerning the subject, and increasing antimycotic resistance.
At the University of Oslo, iGEM is offered as a two-semester course (course code: MBV4910IGEM) and is evaluated on a pass or fail basis. There are no supporting course materials, for this course as the course consists of a student-driven interdisciplinary project in synthetic biology. The course starts in March and finishes in October after the Giant Jamboree. We started brainstorming in the beginning of March and had a clear project idea at the end of March. In April, we started planning the lab work and experiments. In late June we received all components we had ordered; thus we were able to start working in the lab.
On this page, we would like to thank everyone who believed in our project and help us in the execution. We could not have come this far without the contribution of the people and companies listed below.
Work done by others
Without whom this project would not have been possible
DIRK LINKE Primary PI, course leader, and head of the Linke Group
KIRSTEN B. HARALDSEN Advisor and project support. Helped us with outreach and human practises
ATHANASIOS SARAGLIADIS Instructor, provided us with invaluable help and advice during our labwork. We are forever grateful
PAUL GRINI Our secondary PI
Thank you for your aid in the lab, and for your help with matters both practical and theoretical
Marcella O. Rydmark Assisted us with difficult techniques, and protein purification
Ragnhild Eskeland Provided us with expert feedback on CRISPR and guide RNA
National Reference Laboratory for Medical Mycology Many thanks to Cecilie T. Andersen and Lonny M. Kløvfjell for giving us a tour of the laboratory, and general advice concerning yeasts
The Leo Group Thank you for your patience and answering our questions
Beata Nadratowska-Wesolowska Provided us with expert feedback on CRISPR and guide RNA
Sandra Lopez-Aviles Provided us with the yeast cells necessary for a proof-of-concept
The Linke Group Thank you for your patience and warm welcome to the labspace
Difficult technique support
Thank you for helping us to perform the difficult techniques
Bård Enger Mathiesen Technical lab assistance, especially with freeze-drying techniques
Johannes Landskron Provided us with access to a flowcytometer, and helped us set it up for the Interlab study
Thank you for helping us reach out to the community
Åslaug Brynhildsen Aided us with public outreach, and how to communicate with media
Elina Melteig Aided us with public outreach, and how to communicate with media
Thank you for your assistance with matters small and large, and for keeping us in motion
Steinar Mortensen Helped us with booking flight and accomodation, as well as shipping items for sequencing
Arnab Sarkar Created a 3-D printed prototype of our detection kit
Katrine Kjenne Design our logo for us
Marius Øgaard Marius and ShareLab sponsored us with tickets for the Cutting Edge festival!
Bruno A. Dzogovic Helped is with the initial process of the Wiki
Heikki Sørum Greatly assisted us with making 3D-structures of our enzymes, and helped develop our prototype
Nadeem Joudeh Made the animation of our detection kit for our presentation
Presentation feedback and coaching
Thank you for your honest feedback and great advice
Åslaug Brynhildsen Gave us practical tips on how to improve our presentation skills and body language
Anne Greulich Provided us with feedback and advice on how to improve our presentation
Yuri van Ekelenburg Helped improving our presentation
Thomas Trunk Helped improving our presentation
Thank you for helping us reach out to the community
Abigail Sison Was our contact person with iGEM headquarters. Abigail was always available to help us and she also kept us informed about important iGEM deadlines.
Ana Sifuentes When we had trouble with the Wiki, Ana was there to aid us and answer our questions
None of our team-members are particularily skilled in either programming or design. Because of this, making a wiki and graphical layout from scratch turned out to be a huge challenge for us. However, with the many free resources on the world wide web and many, many hours of work, we did it. We are proud of our results, and hope to share them with other iGEM teams. Our code is free to be copied and used for future wikis.
w3schools w3schools.com is a free resource for tutorials and a reference guide for web development. We wrote our wiki by ourselves, and this free resource helped us a lot
Skeleton Skeleton is an open-source boilerplate written by Dave Gamache. It has helped us maintain the typography and scaling of elements on our website. With only ~400 lines of code, it's light weight makes it perfect for smaller projects like this. You can find more information at getskeleton.com