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− | <title> | + | <title>Nanjing-China2018</title> |
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Nanjing-China/CSS:1?action=raw&ctype=text/css" /> | ||
+ | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Nanjing-China/CSS:loader?action=raw&ctype=text/css" /> | ||
<style type="text/css"> | <style type="text/css"> | ||
− | + | --> | |
− | # | + | #loader { |
− | + | position: fixed; | |
− | + | width: 100%; | |
+ | height:100%; | ||
+ | z-index: 20; | ||
+ | display:block; | ||
+ | background-color:rgba(255,255,255,0.5); | ||
+ | |||
+ | } | ||
+ | .spinner { | ||
+ | margin: 100px auto; | ||
+ | width: 100px; | ||
+ | height: 100px; | ||
+ | position: fixed; | ||
} | } | ||
− | + | .container1 > div, .container2 > div, .container3 > div { | |
− | + | width: 30px; | |
− | + | height: 30px; | |
− | + | background-color: #333; | |
− | + | border-radius: 100%; | |
+ | position: absolute; | ||
+ | -webkit-animation: bouncedelay 1.2s infinite ease-in-out; | ||
+ | animation: bouncedelay 1.2s infinite ease-in-out; | ||
+ | -webkit-animation-fill-mode: both; | ||
+ | animation-fill-mode: both; | ||
} | } | ||
− | + | .spinner .spinner-container { | |
− | + | position: absolute; | |
− | + | width: 100%; | |
− | + | height: 100%; | |
− | + | ||
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} | } | ||
− | + | .container2 { | |
− | + | -webkit-transform: rotateZ(45deg); | |
− | + | transform: rotateZ(45deg); | |
− | + | ||
} | } | ||
− | . | + | .container3 { |
− | + | -webkit-transform: rotateZ(90deg); | |
− | + | transform: rotateZ(90deg); | |
− | + | ||
− | + | ||
} | } | ||
− | . | + | .circle1 { top: 0; left: 0; } |
− | + | .circle2 { top: 0; right: 0; } | |
− | + | .circle3 { right: 0; bottom: 0; } | |
− | + | .circle4 { left: 0; bottom: 0; } | |
− | + | .container2 .circle1 { | |
− | + | -webkit-animation-delay: -1.1s; | |
+ | animation-delay: -1.1s; | ||
} | } | ||
− | . | + | |
− | + | .container3 .circle1 { | |
− | + | -webkit-animation-delay: -1.0s; | |
− | + | animation-delay: -1.0s; | |
− | + | } | |
− | + | .container1 .circle2 { | |
− | + | -webkit-animation-delay: -0.9s; | |
− | + | animation-delay: -0.9s; | |
− | + | } | |
+ | .container2 .circle2 { | ||
+ | -webkit-animation-delay: -0.8s; | ||
+ | animation-delay: -0.8s; | ||
} | } | ||
− | . | + | .container3 .circle2 { |
− | + | -webkit-animation-delay: -0.7s; | |
+ | animation-delay: -0.7s; | ||
} | } | ||
− | . | + | .container1 .circle3 { |
− | + | -webkit-animation-delay: -0.6s; | |
− | + | animation-delay: -0.6s; | |
− | + | } | |
+ | .container2 .circle3 { | ||
+ | -webkit-animation-delay: -0.5s; | ||
+ | animation-delay: -0.5s; | ||
} | } | ||
− | + | .container3 .circle3 { | |
− | + | -webkit-animation-delay: -0.4s; | |
− | + | animation-delay: -0.4s; | |
− | + | ||
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} | } | ||
− | + | .container1 .circle4 { | |
− | + | -webkit-animation-delay: -0.3s; | |
− | + | animation-delay: -0.3s; | |
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} | } | ||
− | + | .container2 .circle4 { | |
− | + | -webkit-animation-delay: -0.2s; | |
− | + | animation-delay: -0.2s; | |
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} | } | ||
− | + | .container3 .circle4 { | |
− | + | -webkit-animation-delay: -0.1s; | |
− | + | animation-delay: -0.1s; | |
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} | } | ||
− | + | @-webkit-keyframes bouncedelay { | |
− | + | 0%, 80%, 100% { -webkit-transform: scale(0.0) } | |
− | + | 40% { -webkit-transform: scale(1.0) } | |
− | . | + | } |
− | + | @keyframes bouncedelay { | |
− | + | 0%, 80%, 100% { | |
− | + | transform: scale(0.0); | |
− | + | -webkit-transform: scale(0.0); | |
− | + | } 40% { | |
+ | transform: scale(1.0); | ||
+ | -webkit-transform: scale(1.0); | ||
+ | } | ||
} | } | ||
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#i-menu .i-menu-button{ | #i-menu .i-menu-button{ | ||
width:250px; | width:250px; | ||
− | + | height:150px; | |
float:left; | float:left; | ||
border:4px double #999; | border:4px double #999; | ||
text-align:center; | text-align:center; | ||
− | background-color:rgba( | + | background-color:rgba(102,204,255,0); |
padding:5px; | padding:5px; | ||
font-size:40px; | font-size:40px; | ||
font-family:"Courier New", Courier, monospace;} | font-family:"Courier New", Courier, monospace;} | ||
#i-menu .i-menu-button ul{ margin: auto; padding:0; list-style:none; width:250px; height:150px; line-height:150px; text-align:center; float:left;} | #i-menu .i-menu-button ul{ margin: auto; padding:0; list-style:none; width:250px; height:150px; line-height:150px; text-align:center; float:left;} | ||
− | #i-menu .i-menu-button ul a:hover{ | + | #i-menu .i-menu-button ul a:hover{ background:rgba(204,0,0,0.2);color:#FFFFFF; height:150px; font-size:42px; padding:0px; |
} | } | ||
#i-menu .i-menu-button ul a{ text-decoration:none; color:#000;} | #i-menu .i-menu-button ul a{ text-decoration:none; color:#000;} | ||
#i-menu ul{ | #i-menu ul{ | ||
− | margin: auto; padding:0; list-style:0; background:rgba( | + | margin: auto; padding:0; list-style:0; background:rgba(204,153,255,0); text-align:center; float:left; list-style:0;} |
#i-menu ul a{ | #i-menu ul a{ | ||
text-decoration: none; | text-decoration: none; | ||
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color:#003; | color:#003; | ||
} | } | ||
− | #i-menu ul a:hover{ background:rgba( | + | #i-menu ul a:hover{ background:rgba(255,51,102,0.5); color:#FFFFFF; width:250px; height:150px; font-family: "Comic Sans MS", cursive; font-size:42px; |
} | } | ||
− | #i-menu ul a#on{ background:rgba( | + | #i-menu ul a#on{ background:rgba(120,153,255,0.7); width:250px; height:150px; |
} | } | ||
</style> | </style> | ||
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<body> | <body> | ||
+ | <div id="loader"> | ||
+ | <div class="spinner"> | ||
+ | <div class="spinner-container container1"> | ||
+ | <div class="circle1"></div> | ||
+ | <div class="circle2"></div> | ||
+ | <div class="circle3"></div> | ||
+ | <div class="circle4"></div> | ||
+ | </div> | ||
+ | <div class="spinner-container container2"> | ||
+ | <div class="circle1"></div> | ||
+ | <div class="circle2"></div> | ||
+ | <div class="circle3"></div> | ||
+ | <div class="circle4"></div> | ||
+ | </div> | ||
+ | <div class="spinner-container container3"> | ||
+ | <div class="circle1"></div> | ||
+ | <div class="circle2"></div> | ||
+ | <div class="circle3"></div> | ||
+ | <div class="circle4"></div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <script type="text/javascript"> | ||
+ | $(window).load(function() { | ||
+ | $("#loader").fadeOut("slow"); | ||
+ | }) | ||
+ | </script> | ||
<div id="d"> | <div id="d"> | ||
<img src="https://static.igem.org/mediawiki/2018/f/fe/T--Nanjing-China--signal-1.jpg" width="100%" onclick="MM_effectBlind('HOME', 1000, '0%', '100%', true)" /> | <img src="https://static.igem.org/mediawiki/2018/f/fe/T--Nanjing-China--signal-1.jpg" width="100%" onclick="MM_effectBlind('HOME', 1000, '0%', '100%', true)" /> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
− | <li><a href="https:// | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li> | ||
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</ul> | </ul> | ||
</li> | </li> | ||
− | <li><a href="https://2018.igem.org/Team:Nanjing-China/ | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">JUDGE</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">Bronze</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">Bronze</a></li> | ||
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<li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></li> | ||
+ | |||
</ul> | </ul> | ||
</li> | </li> | ||
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</div> | </div> | ||
<div class="word" id="i-menu" align="center"> | <div class="word" id="i-menu" align="center"> | ||
− | <div class="i-menu-button"><ul><a href="https:// | + | <div class="i-menu-button"><img src="https://static.igem.org/mediawiki/2018/f/fe/T--Nanjing-China--signal-1.jpg" height="150px" /></div> |
− | <div class="i-menu-button"><ul><a href="https://2018.igem.org/Team:Nanjing-China/ | + | <div class="i-menu-button"><ul><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a></ul></div> |
+ | <div class="i-menu-button"><ul><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">JUDGE</a></ul></div> | ||
<div class="i-menu-button"><ul><a href="https://2018.igem.org/Team:Nanjing-China/Design">PROJECT</a></ul></div> | <div class="i-menu-button"><ul><a href="https://2018.igem.org/Team:Nanjing-China/Design">PROJECT</a></ul></div> | ||
<div class="i-menu-button"><ul><a href="https://2018.igem.org/Team:Nanjing-China/Team">TEAM</a></ul></div> | <div class="i-menu-button"><ul><a href="https://2018.igem.org/Team:Nanjing-China/Team">TEAM</a></ul></div> | ||
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</div> | </div> | ||
</div> | </div> | ||
+ | |||
</body> | </body> | ||
</html> | </html> |
Revision as of 04:57, 23 August 2018
Introduction
Light-driven nitrogen fixation system by whole-cell Our team Nanjing-China 2018 intends to establish a sound and ideal whole-cell photocatalytic nitrogen fixation system using E. coli cells engineered with certain proteins and inorganic materials. Thus, the engineered microorganisms that express nitrogenase as well as have the capability of in situ biosynthesis of CdS nanocrystals for the existence of the surface-displayed heavy lead-specific binding proteins is developed. Such system is able to reduce N2 to NH3 driven by light instead of ATP-hydrolysis with considerably high efficiency. The accomplishment of this shall ease the heavy burden of the environment and contribute to the further and better utilization of clean solar energy.