Outside of the team roster

Göttingen Genomics Laboratory

RNA sequencing

For the RNA sequencing analyses, we asked Prof. Dr. Rolf Daniel and Dr. Anja Poehlein from Göttingen Genomics Laboratory (G2L) for support. The G2L delivered comprehensive RNA sequencing data that helped us to develop a glyphosate reporter system.

ITC measurements

For isothermal titration calorimetry (ITC) measurements and structure elucidation of the purified EPSP synthases from E. coli and B. subtilis we sent our proteins to the Bange lab in Marburg. Prof. Dr. Gert Bange is again supervising an iGEM team, which is acknowledged for collaborating.

Seqlab Göttingen

DNA sequencing

To acquire DNA sequences, we sent our samples as described in the notebook section to Microsynth Seqlab, based in Göttingen.

Detection of glyphosate

For the detection of glyphosate, we sent our samples to the Ischebeck lab in Göttingen, which provided us with the possibility to use their gas chromatography and mass spectrometry devices for determining the concentration of glyphosate in samples. TO DO

Genes

We ordered some genes, called g-Blocks, from Integrated DNA Technologies, which was free, since they sponsor the iGEM competition.

Contributions from the lab members

We want to thank Sabine Lentes for enduring eight people in the lab with many questions. She helped us in every situation and was a great aid.
Furthermore, we want to thank Johannes Gibhardt for his help with any technical devices that we used. Especially he is an expert for the platereader.
Christina Herzberg supported us with strains and plasmids, knowledge about technical methods, and answered many questions.
Cedric Blötz helped us with his experience of being a former iGEM participant and for helpful discussions regarding our project.
Anika Klewinghelped us with buffers, kits and chemicals that we needed every day.
Last but not least we want to thank all members from the Stülke lab for their support and endorsement in the time we used their lab and blocked technical devices with our experiments.

Work from team roster

We did all of the lab work by ourselfs as described in the notebook section. The experiments were developed in teamwork with our supervisors.

The over all design was drafted by ourselves. Also, the wiki and website were constructed by us.

Supervisors

The whole project is based on the ideas from Dr. Fabian Commichau that came up to his mind on a train ride to Brussels. We want to thank him for his infectious enthusiasm towards microbiology and great help with each question!

We want to thank also Prof. Dr. Jörg Stülke for kindly providing us with a laboratory, which is obviously necessary for such a project. Furthermore, he greatly supported us with the funding and his experience in leading former iGEM teams.

Construction of strains

We want to thank Dr. Fabian M. Commichau and Sabine Lentes for help with the construction of the deletion strains that were needed for our work.