Difference between revisions of "Team:Goettingen/Attributions"
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<h3>DNA sequencing</h3> | <h3>DNA sequencing</h3> | ||
| − | <p> | + | <p>The best thing that can happen to a DNA: to acquire DNA sequences, we sent our samples to the <em>Microsynth Seqlab</em> sequencing laboratories in Göttingen. |
</p> | </p> | ||
<h3 class="article_clear-right"> | <h3 class="article_clear-right"> | ||
Detection of glyphosate </h3> | Detection of glyphosate </h3> | ||
| − | <p>For the detection of glyphosate, we sent our samples to the <strong>Ischebeck | + | <p>For the detection of glyphosate, we sent our samples to the lab from <strong> Dr. Till Ischebeck</strong> in the Plant Biochemistry Department from the University of Göttingen. The samples were analyzed by GC/MS. |
</p> | </p> | ||
<h3>Genes</h3> | <h3>Genes</h3> | ||
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<h3>Contributions from the lab members</h3> | <h3>Contributions from the lab members</h3> | ||
| − | <p>We | + | <p>We thank <strong>Sabine Lentes</strong> for enduring eight people in the lab with many questions. She helped us in every situation and was a great aid.<br /> Furthermore, we thank <strong>Johannes Gibhardt</strong> for his help to show us the technical devices. Especially he is an expert for the platereader.<br /> <strong>Christina Herzberg</strong> supported us with strains and plasmids, knowledge about technical methods, and answered > 1000 questions.<br /> <strong>Cedric Blötz</strong> helped us with his experience of being a former iGEM participant.<br /> |
| − | <strong>Anika Klewing</strong> | + | <strong>Anika Klewing</strong> supported us with buffers, kits and chemicals that we needed in the lab.<br /> Last but not least, we want to thank all members from the Stülke lab for their support and endorsement during the time we used the lab and blocked technical devices with our experiments. |
</p> | </p> | ||
<h2>Work from team roster</h2> | <h2>Work from team roster</h2> | ||
| − | <p>We did all of the lab work by ourselfs as described in the notebook section. The experiments were developed in teamwork with our supervisors. | + | <p>We did all of the lab work by ourselfs as described in the notebook section. The experiments were developed in teamwork with our supervisors. The over all design was drafted by ourselves. Also, the wiki site and website were constructed on our own. |
| − | + | ||
</p> | </p> | ||
<h3>Supervisors</h3> | <h3>Supervisors</h3> | ||
Revision as of 12:16, 30 August 2018
Team Göttingen
iGEM 2018
Glyphosate on my plate?
| E-mail: | igem2018@gwdg.de |
|---|---|
| Adress: | c/o iGEM 2018 |
| Department of General Microbiology | |
| Grisebachstraße 8 | |
| 37077 Göttingen | |
| Germany |
We are especially grateful to our numerous sponsors on gofundme, who supported our project and made it possible.
Every public donor, who helped us to achieve our goals got a special place on our bacterial wall of fame and a handdrawn microbe to show our appreciation. A link to that wall can be found here:
Attributions
Outside of the team roster
Göttingen Genomics Laboratory
RNA sequencing
For the RNA sequencing analyses, we asked Prof. Dr. Rolf Daniel and Dr. Anja Poehlein from Göttingen Genomics Laboratory (G2L) for support. The G2L delivered comprehensive RNA sequencing data that helped us to develop a glyphosate reporter system.
ITC measurements
For isothermal titration calorimetry (ITC) measurements and structure elucidation of the purified EPSP synthases from E. coli and B. subtilis we sent our proteins to the Bange lab in Marburg. Prof. Dr. Gert Bange is again supervising an iGEM team, which is acknowledged for collaborating.
Seqlab Göttingen
DNA sequencing
The best thing that can happen to a DNA: to acquire DNA sequences, we sent our samples to the Microsynth Seqlab sequencing laboratories in Göttingen.
Detection of glyphosate
For the detection of glyphosate, we sent our samples to the lab from Dr. Till Ischebeck in the Plant Biochemistry Department from the University of Göttingen. The samples were analyzed by GC/MS.
Genes
We ordered some genes, called g-Blocks, from Integrated DNA Technologies, which was free, since they sponsor the iGEM competition.
Contributions from the lab members
We thank Sabine Lentes for enduring eight people in the lab with many questions. She helped us in every situation and was a great aid.
Furthermore, we thank Johannes Gibhardt for his help to show us the technical devices. Especially he is an expert for the platereader.
Christina Herzberg supported us with strains and plasmids, knowledge about technical methods, and answered > 1000 questions.
Cedric Blötz helped us with his experience of being a former iGEM participant.
Anika Klewing supported us with buffers, kits and chemicals that we needed in the lab.
Last but not least, we want to thank all members from the Stülke lab for their support and endorsement during the time we used the lab and blocked technical devices with our experiments.
Work from team roster
We did all of the lab work by ourselfs as described in the notebook section. The experiments were developed in teamwork with our supervisors. The over all design was drafted by ourselves. Also, the wiki site and website were constructed on our own.
Supervisors
The whole project is stimulated by the ideas from PD Dr. Fabian M. Commichau that came up in his mind during a train ride to Brussels. We want to thank him for his infectious enthusiasm for microbiology and his help to solve problems!
We thank Prof. Dr. Jörg Stülke for kindly providing us with lab space, which is essential for a research project. Furthermore, he greatly supported us with funding and his experience as a leader of the iGEM team of the University Göttingen in 2013.
Construction of strains
We are grateful to Sabine Lentes and PD. Dr. Fabian M. Commichau for the construction of B. subtilis mutants.