Difference between revisions of "Team:UCopenhagen/Notebook"

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{{UCopenhagen}}
 
{{UCopenhagen}}
 
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<h1> Lab notebook </h1>
  
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The notebook is a chronological overview of what has happened in the lab. It is organized after each experiment.
  
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<h2>Cloning</h2>
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<h2>Making BioBricks</h2>
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<h2>Liposome characterization</h2>
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<h2>Onion characterization</h2>
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<h2>Leakiness characterization</h2>
  
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<h1>Notebook</h1>
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<h2>Membranes and microscope</h2>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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</div>
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<h3>Week 28 </h3>
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<h4>Learning to make liposomes – 12/07/18</h4>
  
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<p>We were introduced to the process of making liposomes by the nice people in Nikos Hatzakis lab, Søren Schmidt-Rasmussen Nielsen and Mette Galsgaard Malle who gave us the Preparing liposomes protocol. We made some tryout liposomes, that were flashed freezed and stored in -20 oC.</p>
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<i>Selma, Sofia and Attila</i>
  
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<h3>Week 34</h3>
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<h4>Transforming SIEC strains with GFP plasmids – 24/08/18</h4>
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<p>To visualize our bacteria in the microscope together with a membrane we needed it transformed with a GFP plasmid with constitutive expression. We used plasmids from the distribution kit that functioned as Test Device 1 (BBa_J36400) and positive control (BBa_I20270) in the InterLab study.
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Transformation as described in Transformation protocol until step 14, the following combinations of strain and plasmid was made:</p>
  
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<h3>What should this page have?</h3>
 
 
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<li>Chronological notes of what your team is doing.</li>
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  <li>SIEC x BBa_J36400</li>
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<li> Brief descriptions of daily important events.</li>
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  <li>SIEC x BBa_I20270</li>
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<li>Pictures of your progress. </li>
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  <li>SIECΔp1 x BBa_J36400</li>
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<li>Mention who participated in what task.</li>
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  <li>SIECΔp1 x BBa_I20270</li>
 
</ul>
 
</ul>
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<p>Plates were made from agar and LB with chloramphenicol (34 μg/mL) (CAM). Plates with bacteria spent 17 hours in the incubator before being kept in fridge.
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Conclusion: hereafter we used the bacteria transformed with BBa_I20270.</p>
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<i>Selma</i>
  
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<div class="highlight decoration_A_full">
 
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<h3>Inspiration</h3>
 
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<p>You can see what others teams have done to organize their notes:</p>
 
  
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<li><a href="https://2014.igem.org/Team:ATOMS-Turkiye/Notebook">2014 ATOMS-Turkiye</a></li>
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<h2>InterLab</h2>
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<li><a href="https://2014.igem.org/Team:Tec-Monterrey/ITESM14_project.html#tab_notebook">2014 Tec Monterrey</a></li>
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<h2>Egg membrane experiment</h2>
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<li><a href="https://2014.igem.org/Team:Kyoto/Notebook/Magnetosome_Formation#title">2014 Kyoto</a></li>
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<li><a href="https://2014.igem.org/Team:Cornell/notebook">2014 Cornell</a></li>
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</ul>
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</div>
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</div>
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{{UCopenhagen/Footer}}

Revision as of 17:03, 12 September 2018

HOME
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Lab notebook

The notebook is a chronological overview of what has happened in the lab. It is organized after each experiment.

Cloning

Making BioBricks

Liposome characterization

Onion characterization

Leakiness characterization

Membranes and microscope

Week 28

Learning to make liposomes – 12/07/18

We were introduced to the process of making liposomes by the nice people in Nikos Hatzakis lab, Søren Schmidt-Rasmussen Nielsen and Mette Galsgaard Malle who gave us the Preparing liposomes protocol. We made some tryout liposomes, that were flashed freezed and stored in -20 oC.

Selma, Sofia and Attila

Week 34

Transforming SIEC strains with GFP plasmids – 24/08/18

To visualize our bacteria in the microscope together with a membrane we needed it transformed with a GFP plasmid with constitutive expression. We used plasmids from the distribution kit that functioned as Test Device 1 (BBa_J36400) and positive control (BBa_I20270) in the InterLab study. Transformation as described in Transformation protocol until step 14, the following combinations of strain and plasmid was made:

  • SIEC x BBa_J36400
  • SIEC x BBa_I20270
  • SIECΔp1 x BBa_J36400
  • SIECΔp1 x BBa_I20270

Plates were made from agar and LB with chloramphenicol (34 μg/mL) (CAM). Plates with bacteria spent 17 hours in the incubator before being kept in fridge. Conclusion: hereafter we used the bacteria transformed with BBa_I20270.

Selma

InterLab

Egg membrane experiment