Difference between revisions of "Team:Nanjing-China/Safety"

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<h1> Safety </h1>
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<div class="spinner">
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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</script>
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<div id="d">
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<ul>
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<img src="https://static.igem.org/mediawiki/2018/f/fe/T--Nanjing-China--signal-1.jpg" width="100%" onclick="MM_effectBlind('HOME', 1000, '0%', '100%', true)" />
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<li>Choosing a non-pathogenic chassis</li>
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<div align="center">
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<div id="HOME">
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<div class="sub">
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<li>Including an "induced lethality" or "kill-switch" device</li>
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">HP</a></li></ul></div>
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</ul>
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<div class="sub">
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></ul></li></div>
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            <ul>
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    <li><a href="#chassis"><font size="-1">Chassis organisms</font></a></li>
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            <li><a href="#Laboratory"><font size="-1">Laboratory safety</font></a></li></ul>
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<div class="sub">
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a></ul></li></div>
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    <div class="sub">
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></ul></li></div>
  
 
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  <li><a href="https://2018.igem.org/Team:Nanjing-China">HOME</a>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China">Introduction</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li>
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</ul>
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    </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a>
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        <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Composite_Part">Composite</a></li>
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</ul>
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            </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">JUDGE</a>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Bronze">Bronze</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Gold">Gold</a></li>
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</ul>
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      </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">PROJECT</a>
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    <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">Notebook</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Device">Device</a></li>
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</ul>
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        </li>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">TEAM</a>
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    <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Introduction</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li>
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</ul>
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    </li>
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    <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">OTHERS</a>
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    <ul>
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        <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">HP</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li>
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            <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a></li>
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                <li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></li>
  
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<h3>Safe Lab Work</h3>
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    </li>
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      </ul>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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  </div>
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  <div class="header"><img src="https://static.igem.org/mediawiki/2018/e/ec/T--Nanjing-China--title-Safty.png" width="100%" >
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<h3>Safe Shipment</h3>
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
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      <h2>Chassis organisms</h2>
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      <p> The organisms we are dealing with are <em>E.coli</em> BL21 or JM109.Both of them are in Risk Group 1 and can cause no disease to  healthy adults.<em> E.coli</em> is the most studied gram-negative bacterium and a  widely-used model organism in synthetic biology.<br />
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        However, the engineered bacteria may pose  some small risks to people&rsquo;s health and the environment if released into the  real world. Therefore, we have strict rules to prevent this from happening. The  waste produced in the lab are periodically collected, sterilized and  categorized by our team members and then recycled by professional chemical  recycling staffs in our institute. Thus, it is unlikely that these genetically  modified organisms can escape the lab.</p>
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    </div>
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    <div class="word"  id="laboratory">
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      <h2>Laboratory safety</h2>
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      <p>Our experiments are mainly in molecular  level, so that the risks are primarily caused by some chemical and molecular  biology reagents. To minimize these risks, we put the dangerous reagents in  proper storage, and carry out special procedures when dealing with them. For  example, cadmium ion, a toxic substance, is involved for CdS precipitation. We rigorously  recycle the sewage containing Cd ion every time handling it.<br />
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        Another safety concern comes from the  inflammable and explosive acetylene we use to test the activity of nitrogenase.  The gas cylinders which contains acetylene are operated appropriately and  inspected regularly by professional security staffs to avoid gas leakage. <br />
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        Training and enforcement<br />
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      All team  members have received systematical biosafety training from relevant courses in  advance. We all possess the skills in waste disposal, accident prevention,  emergency handling and so on. During our experiments, we stringently observed  the biosafety guidelines issued by our university and WHO. For example, we  ensure electrical safety by connecting wires in proper way and turning off all  the power switches before leaving the lab.</p>
 
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        <div class="f-word"><p><strong><u>Address</u></strong></p>
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            <p>Life Science Department<br />
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              #163 Xianlin Blvd, Qixia District<br />
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              Nanjing University<br />
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              Nanjing, Jiangsu Province<br />
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              P.R. of China<br />
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              Zip: 210046
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            </p></div>
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          <div class="f-word">
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            <p><strong><u>Email</u></strong></p>
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            <p>nanjing_china@163.com</p>
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            <p><strong><u>Social media</u></strong></p>
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            <p>Twitter button: <br/>
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            <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p>
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        <div class="f-img">
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          <div align="center"><img src="https://static.igem.org/mediawiki/2017/0/04/T-Nanjing-China-%E5%8D%97%E4%BA%AC%E5%A4%A7%E5%AD%A6.png" name="img1" width="80" height="110" id="img1" /></div>
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            <div align="right"><img src="https://static.igem.org/mediawiki/2017/d/dc/T-Nanjing-China-%E5%9B%BE%E7%89%872.png" name="img4" width="80" height="80" id="img4"  /></div>
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      <div class="f-b"><img src="https://static.igem.org/mediawiki/2018/a/ad/T--Nanjing-China--footer-1.jpg" width="100%" /></div>
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Revision as of 05:55, 17 September 2018

Nanjing-China2018

Chassis organisms

The organisms we are dealing with are E.coli BL21 or JM109.Both of them are in Risk Group 1 and can cause no disease to healthy adults. E.coli is the most studied gram-negative bacterium and a widely-used model organism in synthetic biology.
However, the engineered bacteria may pose some small risks to people’s health and the environment if released into the real world. Therefore, we have strict rules to prevent this from happening. The waste produced in the lab are periodically collected, sterilized and categorized by our team members and then recycled by professional chemical recycling staffs in our institute. Thus, it is unlikely that these genetically modified organisms can escape the lab.

Laboratory safety

Our experiments are mainly in molecular level, so that the risks are primarily caused by some chemical and molecular biology reagents. To minimize these risks, we put the dangerous reagents in proper storage, and carry out special procedures when dealing with them. For example, cadmium ion, a toxic substance, is involved for CdS precipitation. We rigorously recycle the sewage containing Cd ion every time handling it.
Another safety concern comes from the inflammable and explosive acetylene we use to test the activity of nitrogenase. The gas cylinders which contains acetylene are operated appropriately and inspected regularly by professional security staffs to avoid gas leakage.
Training and enforcement
All team members have received systematical biosafety training from relevant courses in advance. We all possess the skills in waste disposal, accident prevention, emergency handling and so on. During our experiments, we stringently observed the biosafety guidelines issued by our university and WHO. For example, we ensure electrical safety by connecting wires in proper way and turning off all the power switches before leaving the lab.

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china