Difference between revisions of "Team:Nanjing-China/Safety"

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       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></ul></li></div>
 
       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></ul></li></div>
 
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     <li><a href="#chassis"><font size="-1">Chassis organisms</font></a></li>
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             <li><a href="#Laboratory"><font size="-1">Laboratory safety</font></a></li></ul>
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             <li><a href="#R&P"><font size="-1">Risk assessment& Precautions</font></a></li></ul>
 
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       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a></ul></li></div>
 
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       <h2>Chassis organisms</h2>
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       <h2>Overview</h2>
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       <p> The organisms we are dealing with are <em>E.coli</em> BL21 or JM109.Both of them are in Risk Group 1 and can cause no disease to  healthy adults.<em> E.coli</em> is the most studied gram-negative bacterium and a widely-used model organism in synthetic biology.<br />
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       <p> Biosafety is the precaution of large-scale loss of biological integrity in terms of ecology and human’s health.<sup>[1]</sup>These precaution mechanisms include conducting regular reviews of the biosafety in laboratory settings,following strict guidelines,employing an ongoing risk management assessment and so on. Downplaying or failing to fulfill such protocols can lead to augmented risk of exposure to biohazards or pathogens.</p>
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        However, the engineered bacteria may pose some small risks to people&rsquo;s health and the environment if released into the real world. Therefore, we have strict rules to prevent this from happening. The waste produced in the lab are periodically collected, sterilized and categorized by our team members and then recycled by professional chemical  recycling staffs in our institute. Thus, it is unlikely that these genetically  modified organisms can escape the lab.</p>
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      <h2>Risk assessment& Precautions:</h2>
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      <p>Our project is to develop a sound whole-cell  photocatalytic nitrogen fixation system, using engineered <em>E.coli</em> BL21 or JM109 as a factory to produce  ammonia.<br />
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We believe that risks are primarily embodied in three  aspects and we have figured out appropriate solutions to all of them. Firstly,  some chemical and molecular biology reagents we use in experiments might be  harmful and noxious. In order to reduce the adverse effect of these reagents on the environment and our health, we put the hazardous reagents in proper  storage, and carry out special procedures when dealing with them. For example,  cadmium ion, a toxic substance, is involved for CdS precipitation. We rigorously  recycle the sewage containing Cd ion every time handling it. <br />
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Another safety concern comes from the inflammable and  explosive acetylene we use to test the activity of nitrogenase. In order to  avoid gas leakage, the gas cylinders which contains acetylene are operated correctly  and inspected regularly by special security staffs.<br />
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Thirdly, although both <em>E.coli</em> BL21 and JM109 are in Risk Group 1 and can cause no  disease to healthy adults, the genetically modified(GM) organisms can potentially pose threats to the welfare of people and the environment if  released to real world. So we have very strict rules to prevent this from happening. The waste produced in the lab are periodically collected, sterilized  and categorized by our team members and then recycled by professional chemical waste recycling companies. These standard procedures guarantee that the GM organisms would never escape from our labs.</p>
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      We have supervisors  to ensure our operation correctness and all team members have  received systematical biosafety training from relevant courses in advance. We  all possess the skills in waste disposal, accident prevention, emergency measures(such as how  to tackle fire, electric leakage and negligent wounds) and so on. During our  experiments, we stringently observed the biosafety guidelines issued both by  our university and WHO.    </div>
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    <p><em>Biosafety and the environment:An introduction to the Cartagena Protocol on Biosafety. GE.03-01836/E. United Nations Environment Programme. p. 8.</em></p>
 
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      <p>Our experiments are mainly in molecular  level, so that the risks are primarily caused by some chemical and molecular  biology reagents. To minimize these risks, we put the dangerous reagents in  proper storage, and carry out special procedures when dealing with them. For  example, cadmium ion, a toxic substance, is involved for CdS precipitation. We rigorously  recycle the sewage containing Cd ion every time handling it.<br />
 
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        Another safety concern comes from the  inflammable and explosive acetylene we use to test the activity of nitrogenase.  The gas cylinders which contains acetylene are operated appropriately and  inspected regularly by professional security staffs to avoid gas leakage. <br />
 
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        Training and enforcement<br />
 
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      All team  members have received systematical biosafety training from relevant courses in  advance. We all possess the skills in waste disposal, accident prevention,  emergency handling and so on. During our experiments, we stringently observed  the biosafety guidelines issued by our university and WHO. For example, we  ensure electrical safety by connecting wires in proper way and turning off all  the power switches before leaving the lab.</p>
 
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Revision as of 06:16, 19 September 2018

Nanjing-China2018

Overview

Biosafety is the precaution of large-scale loss of biological integrity in terms of ecology and human’s health.[1]These precaution mechanisms include conducting regular reviews of the biosafety in laboratory settings,following strict guidelines,employing an ongoing risk management assessment and so on. Downplaying or failing to fulfill such protocols can lead to augmented risk of exposure to biohazards or pathogens.

Risk assessment& Precautions:

Our project is to develop a sound whole-cell photocatalytic nitrogen fixation system, using engineered E.coli BL21 or JM109 as a factory to produce ammonia.
We believe that risks are primarily embodied in three aspects and we have figured out appropriate solutions to all of them. Firstly, some chemical and molecular biology reagents we use in experiments might be harmful and noxious. In order to reduce the adverse effect of these reagents on the environment and our health, we put the hazardous reagents in proper storage, and carry out special procedures when dealing with them. For example, cadmium ion, a toxic substance, is involved for CdS precipitation. We rigorously recycle the sewage containing Cd ion every time handling it.
Another safety concern comes from the inflammable and explosive acetylene we use to test the activity of nitrogenase. In order to avoid gas leakage, the gas cylinders which contains acetylene are operated correctly and inspected regularly by special security staffs.
Thirdly, although both E.coli BL21 and JM109 are in Risk Group 1 and can cause no disease to healthy adults, the genetically modified(GM) organisms can potentially pose threats to the welfare of people and the environment if released to real world. So we have very strict rules to prevent this from happening. The waste produced in the lab are periodically collected, sterilized and categorized by our team members and then recycled by professional chemical waste recycling companies. These standard procedures guarantee that the GM organisms would never escape from our labs.

      We have supervisors to ensure our operation correctness and all team members have received systematical biosafety training from relevant courses in advance. We all possess the skills in waste disposal, accident prevention, emergency measures(such as how to tackle fire, electric leakage and negligent wounds) and so on. During our experiments, we stringently observed the biosafety guidelines issued both by our university and WHO.

Biosafety and the environment:An introduction to the Cartagena Protocol on Biosafety. GE.03-01836/E. United Nations Environment Programme. p. 8.

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

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