Difference between revisions of "Team:Nanjing-China/Basic Part"

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Revision as of 06:51, 19 September 2018

Nanjing-China2018

Basic_parts
Part number Part name Natural function of part
BBa_K2740001 CR1 Pnif CR1 Pnif is a σ70 (σA)-dependent promoter located upstream of CR1 nifB(BBa_K2740002), which is negatively regulated in response to oxygen and fixed nitrogen.
BBa_K2740002 CR1 nifB CR1 nifB is essential for nitrogen fixation. If the CR1 nifB was deleted, acetylene reduction have not been detected, which meant the nitrogen fixation not happened.
BBa_K2740003 CR1 nifH This part codes Fe protein which is an electron donor to the MoFe protein attributing to the electron delivery in the nitrogen fixation system.
BBa_K2740004 CR1 nifD CR1 nifD codes MoFe protein which is an α2β2 heterotetramer together with CR1 nifK(BBa_K2740004). And the enzyme is responsible for fixing-nitrogen.
BBa_K2740005 CR1 nifK CR1 nifK codes MoFe protein which is an α2β2 heterotetramer together with CR1 nifD(BBa_K2740003). And the enzyme encoded by CR1 nifK is essential for fixing-fixation.
BBa_K2740006 CR1 nifE CR1 nifE contributes to the synthesis and insertion of FeMo-co into nitrogenase together with CR1 nifN. And the FeMo-co in the nitrogenase is quite for maintain its function.
BBa_K2740007 CR1 nifN CR1 nifN contributes to the synthesis and insertion of FeMo-co into nitrogenase.
BBa_K2740008 CR1 nifX CR1 nifX contributes to the synthesis and insertion of FeMo-co into nitrogenase
BBa_K2740009 CR1 HesA CR1 hesA The gene hesA encodes a NAD/FAD-binding protein involved in molybdopterin and thiamine biosynthesis.
BBa_K2740010 CR1 nifV CR1 nifV contributes to synthesizing the homocitrate moiety of FeMo-co which is essential for the nitrogen fixation.
BBa_K2740011 CR1 Tnif CR1 Tnif is a specific terminator for the whole nif gene cluster.