Difference between revisions of "Team:CCU Taiwan/Safety"

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             <div class="container">
 
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                     <li class="title" id="Home"><a>Home</a>
+
 
                         <ul class="sub" id="sub_home">
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                     <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a><hr>
 +
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                             <li class="list" id="home1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Team">Team</a></li>
 
                             <li class="list" id="home1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Team">Team</a></li>
 
<li class="list" id="home2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions">Attributions</a></li>
 
<li class="list" id="home2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions">Attributions</a></li>
 +
<li class="list" id="home2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Medal">Medal</a></li>
 
<li class="list" id="home3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Judge">For Judge</a></li>
 
<li class="list" id="home3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Judge">For Judge</a></li>
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<div id="home_word">
 
<div id="home_word">
<p>There's a home for us, always.</p>
+
<a>There's a home for us, always.</a>
 
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</div>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
                     <li class="title" id="Project"><a href="#">Project</a>
+
                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr>
<ul class="sub" id="sub_project">
+
<ul class="sub" id="sub_project" style="cursor:default;">
 
    <li class="list" id="project1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
 
    <li class="list" id="project1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
 
<li class="list" id="project2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Design">Design</a></li>
 
<li class="list" id="project2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Design">Design</a></li>
 
<li class="list" id="project3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
 
<li class="list" id="project3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
<li class="list" id="project4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements">Achievements</a></li>
+
<li class="list" id="project6"><a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstration</a></li>
 
<li class="list" id="project5"><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab ">InterLab</a></li>
 
<li class="list" id="project5"><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab ">InterLab</a></li>
    <li class="list" id="project6"><a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstration</a></li>
+
<hr>
 
</ul>
 
</ul>
 
</li>
 
</li>
                     <li class="title" id="Parts"><a href="#">Parts</a>
+
                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr>
                         <ul class="sub" id="sub_parts">
+
                         <ul class="sub" id="sub_parts" style="cursor:default;">
    <li class="list" id="parts1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Parts">Parts</a></li>
+
    <li class="list" id="parts1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Parts">Overview</a></li>
 
<li class="list" id="parts2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part">Basic Part</a></li>
 
<li class="list" id="parts2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part">Basic Part</a></li>
 
<li class="list" id="parts3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part">Composite Part</a></li>
 
<li class="list" id="parts3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part">Composite Part</a></li>
 
<li class="list" id="parts4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Improve">Improved Part</a></li>
 
<li class="list" id="parts4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Improve">Improved Part</a></li>
 +
<hr>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
                     <li class="title" id="Modeling"><a href="#">Modeling</a>
+
                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr>
                         <ul class="sub" id="sub_modeling">
+
                         <ul class="sub" id="sub_modeling" style="cursor:default;">
                             <li class="list" id="model1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Model</a></li>
+
                             <li class="list" id="model1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Overview</a></li>
                             <li class="list" id="model2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Enzyme">Enzyme Activity</a></li>
+
                             <li class="list" id="model2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Enzyme">Enzyme Model</a></li>
<li class="list" id="model3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Monolignol">Monolignol Binding</a></li>
+
<li class="list" id="model3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Lignin">Lignin Model</a></li>
 
<li class="list" id="model4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Tools">Tools</a></li>
 
<li class="list" id="model4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Tools">Tools</a></li>
 +
<hr>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
                     <li class="title" id="Drylab"><a href="#">Drylab</a>
+
                     <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Drylab</a><hr>
                         <ul class="sub" id="sub_drylab">
+
                         <ul class="sub" id="sub_drylab" style="cursor:default;">
                             <li class="list" id="drylab1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan">Our Plan</a></li>
+
                             <li class="list" id="drylab1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan">Overview</a></li>
 
<li class="list" id="drylab2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering">Engineering</a></li>
 
<li class="list" id="drylab2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering">Engineering</a></li>
 
<li class="list" id="drylab3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
 
<li class="list" id="drylab3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
 +
<hr>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
<li class="title" id="Human_Practice"><a href="#">Human Practice</a>
+
    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr>
                         <ul class="sub" id="sub_human_practice">
+
                         <ul class="sub" id="sub_human_practice" style="cursor:default;">
                             <li class="list" id="human_practice1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices">Human Practice</a></li>
+
                             <li class="list" id="human_practice1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices">Overview</a></li>
<li class="list" id="human_practice2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engagement">Engagement</a></li>
+
<li class="list" id="human_practice2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engagement">Public</a></li>
 
<li class="list" id="human_practice3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
 
<li class="list" id="human_practice3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
<li class="list" id="human_practice4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Business_Model">Business Model</a></li>
+
<li class="list" id="human_practice4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate">Intergrate</a></li>
 +
<hr>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
<li class="title" id="Notebook"><a href="#">Notebook</a>
+
    <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a>
                         <ul class="sub" id="sub_notebook">
+
                         <ul class="sub" id="sub_notebook" style="cursor:default;">
                             <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
+
                             <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Overview</a></li>
<li class="list" id="notebook2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design">Applied Design</a></li>
+
<li class="list" id="notebook2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations">Collaborations</a></li>
 
<li class="list" id="notebook3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
 
<li class="list" id="notebook3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
 
<li class="list" id="notebook4"><a href="https://2018.igem.org/Team:CCU_Taiwan/DATABASE">DATABASE</a></li>
 
<li class="list" id="notebook4"><a href="https://2018.igem.org/Team:CCU_Taiwan/DATABASE">DATABASE</a></li>
 +
<hr>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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             </div>
 
         </nav>
 
         </nav>
 +
<!--<div class="scroll-line"></div>
 +
<div class="scroll-line-bg"></div>
 +
<div class="Quick-navigation">
 +
  <a href="#A" class="Quick-navigation-item">____Background</a>
 +
  <a href="#B" class="Quick-navigation-item">____Calibration 1</a>
 +
  <a href="#C" class="Quick-navigation-item">____Calibration 2</a>
 +
  <a href="#D" class="Quick-navigation-item">____Calibration 3</a>
 +
  <a href="#E" class="Quick-navigation-item">____Calibration 4</a>
 +
  <a href="#F" class="Quick-navigation-item">____Calibration 5</a>
 +
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 +
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     </header>
 
     </header>
 +
<div class="background">
 +
<div class="bcg"></div>
 +
      <div class="content">
 +
<p class="first">Safety</p>
 +
<p class="second">All members</p>
 +
<p class="description">All of our team members have basic experimental operation exercises and have a basic understanding of the experiment. In addition, all team members attended experimental safety lectures held by our school, we all have certain concepts for laboratory safety.</p>
 +
<p class="second">Wet Lab</p>
 +
<p class="description">In the biological experiment part, the methanolic yeast and Escherichia coli were used, and the experimental contents were all in the first level of Biosafety Level l lab. The operation sites were all in the aseptic operation table, and the personnel were in the experiment. Gloves were worn during the period and equipment were all disinfected with alcohol before and after the experiment.</p>
 +
<p class="second">Dry Lab</p>
 +
<p class="description">In the dry experimental group, we conducted experiments that adhered to laboratory safety rules (such as wearing gloves, using volatile drugs in the draft cabinet, etc.) and having relevant professional guidance. Before using the instrument, the yeast will be filtered out in the laboratory to prevent the yeast from leaving the laboratory. All reactions in the design of the future production line will be carry out in the fermentation tank. Keep the reaction environment in a sealed state, then filtering out the polymer. By two protective measures, filter and heat, we can prevent any leakage. For the filter, we will be using a 33kDa filter, the yeast will not pass. As for the heater, all products will be heated 110 ° C for 10 minutes.</p>
 +
 +
      </div>
 +
</div>
 
</body>
 
</body>
 
 
<div class="column full_size">
 
 
<h1> Safety </h1>
 
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
 
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
 
</div>
 
 
 
<div class="column two_thirds_size">
 
<h3>Safe Project Design</h3>
 
 
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
 
 
<ul>
 
<li>Choosing a non-pathogenic chassis</li>
 
<li>Choosing parts that will not harm humans / animals / plants</li>
 
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
 
<li>Including an "induced lethality" or "kill-switch" device</li>
 
</ul>
 
 
</div>
 
 
<div class="column third_size">
 
<h3>Safe Lab Work</h3>
 
 
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
 
 
<h3>Safe Shipment</h3>
 
 
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
</div>
 
 
  
 
</html>
 
</html>

Revision as of 10:40, 21 September 2018

Safety

All members

All of our team members have basic experimental operation exercises and have a basic understanding of the experiment. In addition, all team members attended experimental safety lectures held by our school, we all have certain concepts for laboratory safety.

Wet Lab

In the biological experiment part, the methanolic yeast and Escherichia coli were used, and the experimental contents were all in the first level of Biosafety Level l lab. The operation sites were all in the aseptic operation table, and the personnel were in the experiment. Gloves were worn during the period and equipment were all disinfected with alcohol before and after the experiment.

Dry Lab

In the dry experimental group, we conducted experiments that adhered to laboratory safety rules (such as wearing gloves, using volatile drugs in the draft cabinet, etc.) and having relevant professional guidance. Before using the instrument, the yeast will be filtered out in the laboratory to prevent the yeast from leaving the laboratory. All reactions in the design of the future production line will be carry out in the fermentation tank. Keep the reaction environment in a sealed state, then filtering out the polymer. By two protective measures, filter and heat, we can prevent any leakage. For the filter, we will be using a 33kDa filter, the yeast will not pass. As for the heater, all products will be heated 110 ° C for 10 minutes.