Difference between revisions of "Team:Nanjing-China/Design"

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       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li></ul></div>
 
       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li></ul></div>
 
             <ul>
 
             <ul>
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     <li><a href="#cds"><font size="-1">CdS semiconductor</font></a></li>
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     <li><a href="#cds">CdS</a></li>
 
   <li><a href="#nitrogen"><font size="-1">Nitrogen fixation</font></a></li>
 
   <li><a href="#nitrogen"><font size="-1">Nitrogen fixation</font></a></li>
 
     <li><a href="#device">Device</a></li>
 
     <li><a href="#device">Device</a></li>
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<div class="word" id="cds">
 
<div class="word" id="cds">
 
<h2>Biosynthesis of CdS semiconductor</h2>
 
<h2>Biosynthesis of CdS semiconductor</h2>
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<p>To construct our light-driven system, we first induce the precipitation of CdS semiconductor on the cell membrane. Two plasmids encoding the surface display protein OmpA-PbrR and the nitrogenase are co-transferred into <em>E. coli</em> strain. After Cd2+ is added into the media, the ions specifically bind to PbrR leading to aggregation of Cd2+ ions. At last when S2- ions are added into the media, <em>E. coli</em> cells for
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<p>To construct our light-driven system, we first induce the precipitation of CdS semiconductor on the cell membrane. Two plasmids encoding the surface display protein OmpA-PbrR and the nitrogenase are co-transferred into <em>E. coli</em> strain. After Cd2+ is added into the media, the ions specifically bind to PbrR leading to aggregation of Cd2+ ions. At last when S2- ions are added into the media, <em>E. coli</em> cells form CdS semiconductor on the cell membrane because of the aggregation.</p></div>
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<div class="word">
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<div  id="design2" style="left:10%; width:80%;">
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<div id="Layer11" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/2/27/T--Nanjing-China--d-1-8.png" width="150" /></div>
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<div id="Layer13" style="visibility: hidden; left: 103px; top: 7px;"><img src="https://static.igem.org/mediawiki/2018/a/a7/T--Nanjing-China--d-2-2.png" width="100" /></div>
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<div id="Layer16" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/2/26/T--Nanjing-China--d-2-4.png" name="Image1" width="202" /></div>
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<div id="Layer17" style="visibility: hidden; left: 245px; top: 196px;"><img src="https://static.igem.org/mediawiki/2018/0/03/T--Nanjing-China--d-2-5.png" width="130" /></div>
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<div id="Layer-all2" style="visibility: visible"><img src="https://static.igem.org/mediawiki/2018/2/29/T--Nanjing-China--d-2-all.png" width="500" /></div>
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<div class="play" onclick="MM_timelinePlay('Timeline2')">play</div>
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<div class="word" id="nitrogen">
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<h2>Light-driven nitrogen fixation in E. coli cells</h2>
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<p>To address the problem of electron transduction, CdS semiconductor  act as semiconductors imitating the photosynthetic system under illumination.  It provided excited electrons to <u>a redox mediator methyl viologen (MV) </u>which  then penetrates into <em>E. coli</em> cells  and transfer the electrons to Mo-Fe protein subunit of nitrogenase. Mo-Fe  protein then utilizes the energy from these electrons to reduce dinitrogen to  ammonia. The semiconductor regains its lost electron from sacrificial electron  donors.<br />
 +
  As a part of biohybrid system, the PbrR protein bears a high  specificity. Our system is supposed to self-repair and can be built with a  rather low cost. This design is of general applications as OmpA protein is only  a surface display machinery for <em>E. coli</em>. <br />
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  This part of the system is the expansion of our hydrogen production,  and it proves that surface display machinery can be expanded to a general  principle for biohybrid photosynthesis.</p>
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  </div>
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<div class="word">
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  <div id="design3" style=" left:10%; width:80%;">
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<div id="Layer21" style="visibility: hidden; left: 0px;"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--Nanjing-China--d-3-8.png" width="240" /></div>
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<div id="Layer22" style="visibility: hidden; top: 19px; left: 30px;"><img src="https://static.igem.org/mediawiki/2018/9/9e/T--Nanjing-China--d-3-7.png" width="170" /></div>
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<div id="Layer23" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/2/21/T--Nanjing-China--d-3-1.png" width="250" /></div>
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<div id="Layer24" style="visibility: hidden; left: 314px; top: 14px;"><img src="https://static.igem.org/mediawiki/2018/f/fd/T--Nanjing-China--d-3-2.png" width="60" /></div>
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<div id="Layer28" style="visibility: hidden"><img src="https://static.igem.org/mediawiki/2018/1/1d/T--Nanjing-China--d-3-5.png" width="150" /></div>
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<div class="play" onclick="MM_timelinePlay('Timeline3')">play</div>
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<div class="word" id="device">
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<h2>Device</h2>
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<p>To apply our system to the real world, we also designed a <a href="https://2018.igem.org/Team:Nanjing-China/Device">device</a> consists of 3 modules: incubation module, illumination module and control module.</p></div>
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</div>
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<div class="footer">
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    <div align="center">
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      <div class="f">
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        <div class="f-word"><p><strong><u>Address</u></strong></p>
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            <p>Life Science Department<br />
 +
              #163 Xianlin Blvd, Qixia District<br />
 +
              Nanjing University<br />
 +
              Nanjing, Jiangsu Province<br />
 +
              P.R. of China<br />
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              Zip: 210046
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            </p></div>
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          <div class="f-word">
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            <p><strong><u>Email</u></strong></p>
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            <p>nanjing_china@163.com</p>
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            <p><strong><u>Social media</u></strong></p>
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            <p>Twitter button: <br/>
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            <a href="https://twitter.com/Guja1501">iGEM Nanjing-China@nanjing_china</a></p>
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            <div align="right"><img src=" https://static.igem.org/mediawiki/2017/d/d4/T-Nanjing-China-%E5%9B%BE%E7%89%873.png
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" name="img2" width="80" height="110" id="img2" /></div>
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          </div>
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          <div class="f-img">
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            <div align="center"><img src="https://static.igem.org/mediawiki/2017/3/37/T-Nanjing-China-s1.png" name="img3" width="80" height="80" id="img3" /></div>
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Revision as of 10:16, 22 September 2018

Nanjing-China2018

Biosynthesis of CdS semiconductor

To construct our light-driven system, we first induce the precipitation of CdS semiconductor on the cell membrane. Two plasmids encoding the surface display protein OmpA-PbrR and the nitrogenase are co-transferred into E. coli strain. After Cd2+ is added into the media, the ions specifically bind to PbrR leading to aggregation of Cd2+ ions. At last when S2- ions are added into the media, E. coli cells form CdS semiconductor on the cell membrane because of the aggregation.

play
stop

Light-driven nitrogen fixation in E. coli cells

To address the problem of electron transduction, CdS semiconductor act as semiconductors imitating the photosynthetic system under illumination. It provided excited electrons to a redox mediator methyl viologen (MV) which then penetrates into E. coli cells and transfer the electrons to Mo-Fe protein subunit of nitrogenase. Mo-Fe protein then utilizes the energy from these electrons to reduce dinitrogen to ammonia. The semiconductor regains its lost electron from sacrificial electron donors.
As a part of biohybrid system, the PbrR protein bears a high specificity. Our system is supposed to self-repair and can be built with a rather low cost. This design is of general applications as OmpA protein is only a surface display machinery for E. coli.
This part of the system is the expansion of our hydrogen production, and it proves that surface display machinery can be expanded to a general principle for biohybrid photosynthesis.

play
stop

Device

To apply our system to the real world, we also designed a device consists of 3 modules: incubation module, illumination module and control module.

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

Social media

Twitter button:
iGEM Nanjing-China@nanjing_china