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{{Goettingen/Main}} | {{Goettingen/Main}} | ||
+ | <html> | ||
+ | <head> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Team:Goettingen/css/notebook?action=raw&ctype=text/css"> | ||
+ | <script src="https://2018.igem.org/Team:Goettingen/js/notebook?action=raw&ctype=text/javascript" type="text/javascript"> | ||
+ | </script> | ||
+ | </head> | ||
+ | <body> | ||
<div class="site-content"> | <div class="site-content"> | ||
− | + | <div class="article"> | |
+ | <div class="article_head-line"> | ||
+ | <h2>Our Labnotes:</h2> | ||
+ | </div> | ||
+ | <div id="notebook-menu"> | ||
+ | <p>In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months. Beyond this section, our strain list is shown.</p> | ||
+ | <div class="notebook-selection_div"> | ||
+ | <div> | ||
+ | <p>Project:</p> | ||
+ | <select class="notebook-select" | ||
+ | name="project"> | ||
+ | <option value="fluorophores">Fluorophores</option> | ||
+ | <option value="biobricks">Biobricks</option> | ||
+ | <option value="evolution">Evolution</option> | ||
+ | <option value="reporterSystems">Reporter-Systems</option> | ||
+ | </select> | ||
+ | </div> | ||
+ | <div> | ||
+ | <p>Month:</p> | ||
+ | <select class="notebook-select" | ||
+ | name="month"> | ||
+ | <option value="April">April</option> | ||
+ | <option value="May">May</option> | ||
+ | <option value="June">June</option> | ||
+ | <option value="July">July</option> | ||
+ | <option value="August">August</option> | ||
+ | <option value="September">September</option> | ||
+ | <option value="October">October</option> | ||
+ | </select> | ||
+ | </div> | ||
+ | </div> | ||
+ | <button id="notebook-menu_submit">Submit</button> | ||
+ | <div style="clear: right"></div> | ||
+ | </div> | ||
+ | <hr id="seperation-ruler"> | ||
+ | <div id="notebook-display"> | ||
+ | |||
+ | |||
+ | <div class="article_table"> | ||
+ | <p><strong>STRAIN LIST</strong></p> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Name</th> | ||
+ | <th>Name after isolation</th> | ||
+ | <th> | ||
+ | <center>Genotype</center> | ||
+ | </th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>168</td> | ||
+ | <td></td> | ||
+ | <td><em>trpC2</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>SP1</td> | ||
+ | <td></td> | ||
+ | <td>wildtype</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM1</td> | ||
+ | <td>Suppressor 1 10 mM</td> | ||
+ | <td><em>trpC2</em> 1.3 kbp deletion <em>5'-yhfE-yhfF-3'-gltT</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM2</td> | ||
+ | <td>Suppressor 2 10 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM3</td> | ||
+ | <td>Suppressor 3 10 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM4</td> | ||
+ | <td>Suppressor 4 10 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM5</td> | ||
+ | <td>Suppressor 5 10 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM6</td> | ||
+ | <td>Suppressor 1-1 30 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM7</td> | ||
+ | <td>Suppressor 1-2 30 mM</td> | ||
+ | <td><em>trpC2</em> 1.3 kbp deletion <em>5'-yhfE-yhfF-3'-gltT gltP</em>(G232A)</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM8</td> | ||
+ | <td>Suppressor 2-1 30 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM9</td> | ||
+ | <td>Suppressor 2-2 30 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM10</td> | ||
+ | <td>Suppressor 3-1 30 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM11</td> | ||
+ | <td>Suppressor 3-2 30 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM12</td> | ||
+ | <td>Suppressor 1-1 40 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM13</td> | ||
+ | <td>Suppressor 1-2 40 mM</td> | ||
+ | <td><em>trpC2</em> 1.3 kbp deletion <em>5'-yhfE-yhfF-3'-gltT</em> 11 bp deletion in <em>gltP</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM14</td> | ||
+ | <td>Suppressor 2-1 40 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM15</td> | ||
+ | <td>Suppressor 2-2 40 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM16</td> | ||
+ | <td>Suppressor 3-1 40 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM17</td> | ||
+ | <td>Suppressor 3-2 40 mM</td> | ||
+ | <td><em>trpC2</em> not determined</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> BP233</td> | ||
+ | <td></td> | ||
+ | <td><em> trpC2</em> Δ<em>gltT::spc</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td> BP234</td> | ||
+ | <td></td> | ||
+ | <td><em>trpC2</em> Δ<em>gltP::cat</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BP235</td> | ||
+ | <td></td> | ||
+ | <td><em> trpC2</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>BP236</td> | ||
+ | <td></td> | ||
+ | <td> <em> trpC2 amyE::(Palf4-gltT-lacZ aphA3)</em> </td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>BP237</td> | ||
+ | <td></td> | ||
+ | <td> <em> trpC2</em> Δ<em>gltT::spc amyE::(Palf4-gltT-lacZ aphA3)</em> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM18</td> | ||
+ | <td></td> | ||
+ | <td><em>amyE::(PtrpE-lacZ aphA3)</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM19</td> | ||
+ | <td></td> | ||
+ | <td><em>trpC2 amyE::(PtrpE-lacZ aphA3)</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM20</td> | ||
+ | <td>SP1_GP342</td> | ||
+ | <td> <em> amyE::(PgltA-lacZ aphA3)</em> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM21</td> | ||
+ | <td>SP1_GP650</td> | ||
+ | <td> <em> amyE::(PgltA-lacZ aphA3) gltC::TnIO spc </em> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM22 </td> | ||
+ | <td>168::BFP</td> | ||
+ | <td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM23 </td> | ||
+ | <td>168::GFP</td> | ||
+ | <td> <em>trpC2 amyE::(GFP-lacZ aphA3) </em></td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>iGEM24</td> | ||
+ | <td>168::mOrange</td> | ||
+ | <td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> Δ<em>gltT::spc </em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM25</td> | ||
+ | <td>168::mCherry</td> | ||
+ | <td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM26</td> | ||
+ | <td>BP233::BFP</td> | ||
+ | <td> <em>trpC2 amyE::(BFP-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM27</td> | ||
+ | <td>BP233::GFP</td> | ||
+ | <td> <em>trpC2 amyE::(GFP-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM28</td> | ||
+ | <td>BP233::mOrange</td> | ||
+ | <td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> Δ<em>gltT::spc </em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM29</td> | ||
+ | <td>BP233::mCherry</td> | ||
+ | <td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM30</td> | ||
+ | <td>Spden::BFP</td> | ||
+ | <td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat </em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM31</td> | ||
+ | <td>Spden::GFP </td> | ||
+ | <td> <em> trpC2 amyE::(GFP-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM32</td> | ||
+ | <td>Spden::mOrange</td> | ||
+ | <td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM33</td> | ||
+ | <td>Spden::mCherry</td> | ||
+ | <td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM34</td> | ||
+ | <td>BP235::BFP</td> | ||
+ | <td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em> </td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM35</td> | ||
+ | <td>BP235::GFP</td> | ||
+ | <td> <em> trpC2 amyE::(GFP-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM36</td> | ||
+ | <td>BP235::mOrange</td> | ||
+ | <td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM37</td> | ||
+ | <td>SP1::Gat</td> | ||
+ | <td><em>amyE::(gat-lacZ aphA3)</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM38</td> | ||
+ | <td>SP1::AroA*</td> | ||
+ | <td><em>amyE::(aroA*-lacZ aphA3)</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM39</td> | ||
+ | <td>SP1::Gat::Δ<em>gltT</em></td> | ||
+ | <td><em>amyE::(gat-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM40</td> | ||
+ | <td>SP1::AroA*::Δ<em>gltT</em></td> | ||
+ | <td><em>amyE::(aroA*-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>iGEM41</td> | ||
+ | <td>SP1::Δ<em>gltT</em></td> | ||
+ | <td>Δ<em>gltT::spc</em></td> | ||
+ | </tr> | ||
+ | </table> | ||
</div> | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
+ | </html> |
Latest revision as of 11:25, 5 October 2018
Team Göttingen
iGEM 2018
Glyphosate on my plate?
Our Labnotes:
STRAIN LIST
Name | Name after isolation |
|
---|---|---|
168 | trpC2 | |
SP1 | wildtype | |
iGEM1 | Suppressor 1 10 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT |
iGEM2 | Suppressor 2 10 mM | trpC2 not determined |
iGEM3 | Suppressor 3 10 mM | trpC2 not determined |
iGEM4 | Suppressor 4 10 mM | trpC2 not determined |
iGEM5 | Suppressor 5 10 mM | trpC2 not determined |
iGEM6 | Suppressor 1-1 30 mM | trpC2 not determined |
iGEM7 | Suppressor 1-2 30 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT gltP(G232A) |
iGEM8 | Suppressor 2-1 30 mM | trpC2 not determined |
iGEM9 | Suppressor 2-2 30 mM | trpC2 not determined |
iGEM10 | Suppressor 3-1 30 mM | trpC2 not determined |
iGEM11 | Suppressor 3-2 30 mM | trpC2 not determined |
iGEM12 | Suppressor 1-1 40 mM | trpC2 not determined |
iGEM13 | Suppressor 1-2 40 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT 11 bp deletion in gltP |
iGEM14 | Suppressor 2-1 40 mM | trpC2 not determined |
iGEM15 | Suppressor 2-2 40 mM | trpC2 not determined |
iGEM16 | Suppressor 3-1 40 mM | trpC2 not determined |
iGEM17 | Suppressor 3-2 40 mM | trpC2 not determined |
BP233 | trpC2 ΔgltT::spc | |
BP234 | trpC2 ΔgltP::cat | |
BP235 | trpC2 ΔgltT::spc ΔgltP::cat | |
BP236 | trpC2 amyE::(Palf4-gltT-lacZ aphA3) | |
BP237 | trpC2 ΔgltT::spc amyE::(Palf4-gltT-lacZ aphA3) | |
iGEM18 | amyE::(PtrpE-lacZ aphA3) | |
iGEM19 | trpC2 amyE::(PtrpE-lacZ aphA3) | |
iGEM20 | SP1_GP342 | amyE::(PgltA-lacZ aphA3) |
iGEM21 | SP1_GP650 | amyE::(PgltA-lacZ aphA3) gltC::TnIO spc |
iGEM22 | 168::BFP | trpC2 amyE::(BFP-lacZ aphA3) |
iGEM23 | 168::GFP | trpC2 amyE::(GFP-lacZ aphA3) |
iGEM24 | 168::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc |
iGEM25 | 168::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc |
iGEM26 | BP233::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc |
iGEM27 | BP233::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc |
iGEM28 | BP233::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc |
iGEM29 | BP233::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc |
iGEM30 | Spden::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
iGEM31 | Spden::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
iGEM32 | Spden::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
iGEM33 | Spden::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
iGEM34 | BP235::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
iGEM35 | BP235::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
iGEM36 | BP235::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
iGEM37 | SP1::Gat | amyE::(gat-lacZ aphA3) |
iGEM38 | SP1::AroA* | amyE::(aroA*-lacZ aphA3) |
iGEM39 | SP1::Gat::ΔgltT | amyE::(gat-lacZ aphA3) ΔgltT::spc |
iGEM40 | SP1::AroA*::ΔgltT | amyE::(aroA*-lacZ aphA3) ΔgltT::spc |
iGEM41 | SP1::ΔgltT | ΔgltT::spc |