Difference between revisions of "Team:Goettingen/Notebook"

 
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        <h2>Our Labnotes:</h2>
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    <h2>Our Labnotes:</h2>
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    </div>
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  </div>
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    <div id="notebook-menu">
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        <button class="notebook-menu-is-active">April</button>
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    <p>In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months. Beyond this section, our strain list is shown.</p>
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         <button>May</button>
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    <div class="notebook-selection_div">
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         <button>June</button>
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      <div>
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        <button>July</button>
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         <p>Project:</p>
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        <button>August</button>
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         <select class="notebook-select"
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        <button>September</button>
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                name="project">
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         <button>October</button>
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          <option value="fluorophores">Fluorophores</option>
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    </div>
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          <option value="biobricks">Biobricks</option>
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    <div class="notebook-dateselect">
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          <option value="evolution">Evolution</option>
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        <h3>April</h3>
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          <option value="reporterSystems">Reporter-Systems</option>
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        <a>25.04.18 &ndash; 30.04.18</a>
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         </select>
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    </div>
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      </div>
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    <div id="notebook-display">
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      <div>
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        <p>Month:</p>
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        <select class="notebook-select"
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                name="month">
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      <option value="April">April</option>
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      <option value="May">May</option>
 +
      <option value="June">June</option>
 +
      <option value="July">July</option>
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      <option value="August">August</option>
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      <option value="September">September</option>
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      <option value="October">October</option>
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    </select>
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      </div>
 
     </div>
 
     </div>
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    <button id="notebook-menu_submit">Submit</button>
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<div class="article_table">
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<p><strong>STRAIN LIST</strong></p>
 +
<table>
 +
<tr>
 +
<th>Name</th>
 +
<th>Name after isolation</th>
 +
<th>
 +
<center>Genotype</center>
 +
</th>
 +
</tr>
 +
<tr>
 +
<td>168</td>
 +
<td></td>
 +
<td><em>trpC2</em></td>
 +
</tr>
 +
<tr>
 +
<td>SP1</td>
 +
<td></td>
 +
<td>wildtype</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM1</td>
 +
<td>Suppressor 1 10 mM</td>
 +
<td><em>trpC2</em> 1.3&nbsp;kbp deletion <em>5'-yhfE-yhfF-3'-gltT</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM2</td>
 +
<td>Suppressor 2 10 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM3</td>
 +
<td>Suppressor 3 10 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM4</td>
 +
<td>Suppressor 4 10 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM5</td>
 +
<td>Suppressor 5 10 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM6</td>
 +
<td>Suppressor 1-1 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM7</td>
 +
<td>Suppressor 1-2 30 mM</td>
 +
<td><em>trpC2</em> 1.3&nbsp;kbp deletion <em>5'-yhfE-yhfF-3'-gltT gltP</em>(G232A)</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM8</td>
 +
<td>Suppressor 2-1 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM9</td>
 +
<td>Suppressor 2-2 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM10</td>
 +
<td>Suppressor 3-1 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM11</td>
 +
<td>Suppressor 3-2 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM12</td>
 +
<td>Suppressor 1-1 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM13</td>
 +
<td>Suppressor 1-2 40 mM</td>
 +
<td><em>trpC2</em> 1.3&nbsp;kbp deletion <em>5'-yhfE-yhfF-3'-gltT</em> 11&nbsp;bp deletion in <em>gltP</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM14</td>
 +
<td>Suppressor 2-1 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM15</td>
 +
<td>Suppressor 2-2 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM16</td>
 +
<td>Suppressor 3-1 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM17</td>
 +
<td>Suppressor 3-2 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td> BP233</td>
 +
<td></td>
 +
<td><em> trpC2</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td> BP234</td>
 +
<td></td>
 +
<td><em>trpC2</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>BP235</td>
 +
<td></td>
 +
<td><em> trpC2</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>BP236</td>
 +
<td></td>
 +
<td> <em> trpC2 amyE::(Palf4-gltT-lacZ aphA3)</em> </td>
 +
</tr>
 +
 +
<tr>
 +
<td>BP237</td>
 +
<td></td>
 +
<td> <em> trpC2</em> &Delta;<em>gltT::spc amyE::(Palf4-gltT-lacZ aphA3)</em> </td>
 +
</tr>
 +
<tr>
 +
<td>iGEM18</td>
 +
<td></td>
 +
<td><em>amyE::(PtrpE-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM19</td>
 +
<td></td>
 +
<td><em>trpC2 amyE::(PtrpE-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM20</td>
 +
<td>SP1_GP342</td>
 +
<td> <em> amyE::(PgltA-lacZ aphA3)</em> </td>
 +
</tr>
 +
<tr>
 +
<td>iGEM21</td>
 +
<td>SP1_GP650</td>
 +
<td> <em> amyE::(PgltA-lacZ aphA3) gltC::TnIO spc </em> </td>
 +
</tr>
 +
<tr>
 +
<td>iGEM22 </td>
 +
<td>168::BFP</td>
 +
<td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM23 </td>
 +
<td>168::GFP</td>
 +
<td> <em>trpC2 amyE::(GFP-lacZ aphA3) </em></td>
 +
</tr>
 +
 +
<tr>
 +
<td>iGEM24</td>
 +
<td>168::mOrange</td>
 +
<td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> &Delta;<em>gltT::spc </em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM25</td>
 +
<td>168::mCherry</td>
 +
<td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM26</td>
 +
<td>BP233::BFP</td>
 +
<td> <em>trpC2 amyE::(BFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM27</td>
 +
<td>BP233::GFP</td>
 +
<td> <em>trpC2 amyE::(GFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM28</td>
 +
<td>BP233::mOrange</td>
 +
<td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> &Delta;<em>gltT::spc </em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM29</td>
 +
<td>BP233::mCherry</td>
 +
<td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM30</td>
 +
<td>Spden::BFP</td>
 +
<td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat </em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM31</td>
 +
<td>Spden::GFP </td>
 +
<td> <em> trpC2 amyE::(GFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM32</td>
 +
<td>Spden::mOrange</td>
 +
<td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM33</td>
 +
<td>Spden::mCherry</td>
 +
<td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM34</td>
 +
<td>BP235::BFP</td>
 +
<td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em> </td>
 +
</tr>
 +
<tr>
 +
<td>iGEM35</td>
 +
<td>BP235::GFP</td>
 +
<td> <em> trpC2 amyE::(GFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM36</td>
 +
<td>BP235::mOrange</td>
 +
<td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM37</td>
 +
<td>SP1::Gat</td>
 +
<td><em>amyE::(gat-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM38</td>
 +
<td>SP1::AroA*</td>
 +
<td><em>amyE::(aroA*-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM39</td>
 +
<td>SP1::Gat::&Delta;<em>gltT</em></td>
 +
<td><em>amyE::(gat-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM40</td>
 +
<td>SP1::AroA*::&Delta;<em>gltT</em></td>
 +
<td><em>amyE::(aroA*-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM41</td>
 +
<td>SP1::&Delta;<em>gltT</em></td>
 +
<td>&Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
</table>
 +
</div>
 +
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Latest revision as of 11:25, 5 October 2018

Our Labnotes:

In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months. Beyond this section, our strain list is shown.

Project:

Month:

STRAIN LIST

Name Name after isolation
Genotype
168 trpC2
SP1 wildtype
iGEM1 Suppressor 1 10 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT
iGEM2 Suppressor 2 10 mM trpC2 not determined
iGEM3 Suppressor 3 10 mM trpC2 not determined
iGEM4 Suppressor 4 10 mM trpC2 not determined
iGEM5 Suppressor 5 10 mM trpC2 not determined
iGEM6 Suppressor 1-1 30 mM trpC2 not determined
iGEM7 Suppressor 1-2 30 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT gltP(G232A)
iGEM8 Suppressor 2-1 30 mM trpC2 not determined
iGEM9 Suppressor 2-2 30 mM trpC2 not determined
iGEM10 Suppressor 3-1 30 mM trpC2 not determined
iGEM11 Suppressor 3-2 30 mM trpC2 not determined
iGEM12 Suppressor 1-1 40 mM trpC2 not determined
iGEM13 Suppressor 1-2 40 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT 11 bp deletion in gltP
iGEM14 Suppressor 2-1 40 mM trpC2 not determined
iGEM15 Suppressor 2-2 40 mM trpC2 not determined
iGEM16 Suppressor 3-1 40 mM trpC2 not determined
iGEM17 Suppressor 3-2 40 mM trpC2 not determined
BP233 trpC2 ΔgltT::spc
BP234 trpC2 ΔgltP::cat
BP235 trpC2 ΔgltT::spc ΔgltP::cat
BP236 trpC2 amyE::(Palf4-gltT-lacZ aphA3)
BP237 trpC2 ΔgltT::spc amyE::(Palf4-gltT-lacZ aphA3)
iGEM18 amyE::(PtrpE-lacZ aphA3)
iGEM19 trpC2 amyE::(PtrpE-lacZ aphA3)
iGEM20 SP1_GP342 amyE::(PgltA-lacZ aphA3)
iGEM21 SP1_GP650 amyE::(PgltA-lacZ aphA3) gltC::TnIO spc
iGEM22 168::BFP trpC2 amyE::(BFP-lacZ aphA3)
iGEM23 168::GFP trpC2 amyE::(GFP-lacZ aphA3)
iGEM24 168::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc
iGEM25 168::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc
iGEM26 BP233::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc
iGEM27 BP233::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc
iGEM28 BP233::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc
iGEM29 BP233::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc
iGEM30 Spden::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM31 Spden::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM32 Spden::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM33 Spden::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM34 BP235::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM35 BP235::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM36 BP235::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM37 SP1::Gat amyE::(gat-lacZ aphA3)
iGEM38 SP1::AroA* amyE::(aroA*-lacZ aphA3)
iGEM39 SP1::Gat::ΔgltT amyE::(gat-lacZ aphA3) ΔgltT::spc
iGEM40 SP1::AroA*::ΔgltT amyE::(aroA*-lacZ aphA3) ΔgltT::spc
iGEM41 SP1::ΔgltT ΔgltT::spc