Difference between revisions of "Team:Goettingen/Notebook"

 
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        <h2>Our Labnotes:</h2>
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    <h2>Our Labnotes:</h2>
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    </div>
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  </div>
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        <button class="notebook-menu-is-active">April</button>
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     <p>In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months. Beyond this section, our strain list is shown.</p>
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        <button>May</button>
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     <div class="notebook-selection_div">
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        <button>June</button>
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      <div>
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        <button>July</button>
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        <p>Project:</p>
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        <button>August</button>
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         <select class="notebook-select"
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        <button>September</button>
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                 name="project">
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        <button>October</button>
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          <option value="fluorophores">Fluorophores</option>
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     </div>
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          <option value="biobricks">Biobricks</option>
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    <div class="notebook-dateselect">
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          <option value="evolution">Evolution</option>
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        <h3>April</h3>
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          <option value="reporterSystems">Reporter-Systems</option>
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        <a>25.04.18 &ndash; 30.04.18</a>
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        </select>
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    </div>
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      </div>
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    <div id="notebook-display">
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      <div>
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    </div>
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         <p>Month:</p>
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</div>
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         <select class="notebook-select"
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</div>
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                 name="month">
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<div class="notebook-item">
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      <option value="April">April</option>
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    <div class="notebook-head">
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      <option value="May">May</option>
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        <h3 class="notebook-head_title">Used recipes for buffers and media
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      <option value="June">June</option>
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        </h3>
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      <option value="July">July</option>
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        <p class="notebook-head_date">25.04.18</p>
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      <option value="August">August</option>
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    </div>
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      <option value="September">September</option>
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     <div class="notebook-content">
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      <option value="October">October</option>
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        <div class="notebook-table article_table">
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    </select>
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            <p>LB-Medium</p>
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      </div>
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            <table>
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                <tr>
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                    <th>Components</th>
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−
                    <th class="article-table_right">Amount (g)</th>
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                </tr>
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                <tr>
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                    <td>Tryptone</td>
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                    <td class="article-table_right">10</td>
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                </tr>
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                <tr>
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                    <td>NaCl</td>
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                    <td class="article-table_right">10</td>
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                </tr>
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                <tr>
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                    <td>Yeast exctract</td>
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                    <td class="article-table_right">5</td>
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                </tr>
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                <tr>
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                    <td>1000 mL H<sub>2</sub>O</td>
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                    <td></td>
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                </tr>
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                <tr class="article_table_hline">
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                    <td><strong>Autoclavation</strong></td>
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                    <td></td>
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                </tr>
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            </table>
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         </div>
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        <div class="notebook-table article_table">
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            <p>SP-Medium</p>
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            <table>
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                 <tr>
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                    <th>Components</th>
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−
                    <th class="article-table_right">Amount (g)</th>
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                </tr>
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                <tr>
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                    <td>Nutrient broth</td>
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                    <td class="article-table_right">8</td>
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                </tr>
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                <tr>
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                    <td>MgS0<sub>4</sub> &#9679; 7 H<sub>2</sub>O</td>
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                    <td class="article-table_right">10</td>
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                </tr>
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                <tr>
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                    <td>KCl</td>
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                    <td class="article-table_right">1</td>
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                </tr>
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                <tr>
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                    <td>1000 mL H<sub>2</sub>O</td>
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                    <td></td>
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                </tr>
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                <tr>
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                    <td><strong>Autoclavation</strong></td>
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                    <td></td>
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                </tr>
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                <tr>
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                    <td>CaCl<sub>2</sub></td>
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                    <td class="article-table_right">0.5&nbsp;M</td>
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                </tr>
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                <tr>
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                    <td>MnCl<sub>2</sub></td>
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                    <td class="article-table_right">10&nbsp;mM</td>
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                </tr>
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                <tr>
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                    <td>Iron ferric ammonium citrate</td>
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                    <td class="article-table_right">2&nbsp;mL</td>
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                </tr>
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            </table>
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        </div>
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         <div class="notebook-table article_table">
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            <p>10&times; MN-Medium</p>
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            <table>
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                <tr>
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−
                    <th>Components</th>
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−
                    <th class="article-table_right">Amount (g)</th>
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                </tr>
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−
                <tr>
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−
                    <td>K<sub>2</sub>HPO<sub>4</sub> &#9679; 3 H<sub>2</sub>O</td>
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−
                    <td class="article-table_right">136</td>
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−
                </tr>
+
−
                <tr>
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−
                    <td>KH<sub>2</sub>PO<sub>4</sub></td>
+
−
                    <td class="article-table_right">60</td>
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                </tr>
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−
                <tr>
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−
                    <td>Na citrate</td>
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−
                    <td class="article-table_right">10</td>
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                </tr>
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−
                <tr>
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−
                    <td>1000 mL H<sub>2</sub>O</td>
+
−
                    <td></td>
+
−
                </tr>
+
−
                <tr class="article_table_hline">
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−
                    <td><strong>Ad to 50 mL with sterile water</strong></td>
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                    <td class="article-table_right">100</td>
+
−
                </tr>
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−
            </table>
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         </div>
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        <div class="notebook-table article_table">
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            <p>CS-Glucose Medium</p>
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            <table>
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−
            <tr>
+
−
                 <th>Components</th>
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−
                <th class="article-table_right">Amount (ml)</th>
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            </tr>
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                <tr>
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−
                    <td>C-Salts</td>
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                    <td class="article-table_right">10</td>
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                </tr>
+
−
                <tr>
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                    <td>III-Salts</td>
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−
                    <td class="article-table_right">0.5</td>
+
−
                </tr>
+
−
                <tr>
+
−
                    <td>Tryptophane</td>
+
−
                    <td class="article-table_right">0.5</td>
+
−
                </tr>
+
−
                <tr>
+
−
                    <td>Na-Succinate</td>
+
−
                    <td class="article-table_right">1</td>
+
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                </tr>
+
−
                <tr>
+
−
                    <td>50 % Glucose</td>
+
−
                    <td class="article-table_right">0.5</td>
+
−
                </tr>
+
−
                <tr class="article_table_hline">
+
−
                    <td><strong>Ad to 50 mL with sterile water</strong></td>
+
−
            <td></td>
+
−
            </table>
+
−
        </div>
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            <div class="notebook-table article_table">
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            <p>CS-Glucose Agar Plates</p>
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−
            <table>
+
−
            <tr>
+
−
                <th>Components</th>
+
−
                <th class="article-table_right">Amount (ml)</th>
+
−
            </tr>
+
−
                <tr>
+
−
                    <td>CS-Glucose Medium</td>
+
−
                    <td class="article-table_right">25</td>
+
−
                </tr>
+
−
                <tr>
+
−
                    <td>3 % Bacto-Agar</td>
+
−
                    <td class="article-table_right">25</td>
+
−
                </tr>
+
−
                </table>
+
−
        </div>
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     </div>
 
     </div>
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    <button id="notebook-menu_submit">Submit</button>
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        <h3 class="notebook-head_title"></h3>
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        <p class="notebook-head_date">27.04.18</p>
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     </div>
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    <div class="notebook-content">
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        <p>Cryo cultures of strains 168, BP233, BP234 were prepared with 90% LB medium and 10% DMSO. The cells were frozen and stored at -80°C.</p>
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    </div>
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<div class="notebook-item">
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    <div class="notebook-head">
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        <h3 class="notebook-head_title"></h3>
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        <p class="notebook-head_date">02.05.18</p>
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    </div>
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    <div class="notebook-content">
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        <p>The strains BP233, BP235 and the WT strain 168 were propagated to 3 ml LB medium and inoculated for 4 h at 37°C and 220 rpm. They were harvested in a 2 ml reaction tube by centrifugation at 13000 rpm for one minute. The supernatant was discared and the cells were washed in 1x C-Salts. Afterwards, they were resuspended in 300 µl 1x C-Salts. The OD<sub>600</sub> was determined.</p>
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                    <div class="notebook-table article_table">
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−
            <table>
+
−
                <tr>
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−
                    <th>Strain</th>
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−
                    <th class="article-table_right">OD<sub>600</sub></th>
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                </tr>
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                <tr>
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                    <td>168</td>
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                    <td class="article-table_right">9.8</td>
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−
                </tr>
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−
                <tr>
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−
                    <td>BP233</td>
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−
                    <td class="article-table_right">6.1</td>
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                </tr>
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−
                <tr>
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                    <td>BP235</td>
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                    <td class="article-table_right">7.6</td>
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−
                </tr>
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−
            </table>
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−
    </div>
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        <p>The cells were then struck out on cs-glucose agar plates with variating glyphosate concentrations. 168 was propagated on plates containing 0 mM and 10 mM glyphosate, while BP233 was additionally propagated on plates with the glyphosate concentrations of 30 mM and 40 mM.BP235 was additonally propagated on plates containing glyphosate concentrations of 50 mM and 60 mM. All agar plates were then inoculated at 37 °C for 2 days.   </p>
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<div class="article_table">
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        <p>The strains 168, BP233 and BP236 were propagated to 3 ml LB medium and inoculated for 4 h at 37°C and 220 rpm. They were harvested in a 2 ml reaction tube by centrifugation at 13000 rpm for one minute. The supernatant was discared and the cells were washed in 1x C-Salts. Afterwards, they were resuspended in 300 µl 1x C-Salts. The OD<sub>600</sub> was determined.</p>  
+
<p><strong>STRAIN LIST</strong></p>
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              <div class="notebook-table article_table">
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<table>
−
            <table>
+
<tr>
−
                <tr>
+
<th>Name</th>
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                    <th>Strain</th>
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<th>Name after isolation</th>
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                    <th class="article-table_right">OD<sub>600</sub></th>
+
<th>
−
                </tr>
+
<center>Genotype</center>
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                <tr>
+
</th>
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                    <td>168</td>
+
</tr>
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                    <td class="article-table_right">10.74</td>
+
<tr>
−
                </tr>
+
<td>168</td>
−
                <tr>
+
<td></td>
−
                    <td>BP233</td>
+
<td><em>trpC2</em></td>
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                    <td class="article-table_right">8.37</td>
+
</tr>
−
                </tr>
+
<tr>
−
                <tr>
+
<td>SP1</td>
−
                    <td>BP236</td>
+
<td></td>
−
                    <td class="article-table_right">7.71</td>
+
<td>wildtype</td>
−
                </tr>
+
</tr>
−
            </table>
+
<tr>
−
    </div>
+
<td>iGEM1</td>
−
 
+
<td>Suppressor 1 10 mM</td>
−
        <p>100 µl of solutions were propagated on CS-Glucose agar plates containing 0 mM glyphosate as well as plates containing 10 mM glyphosate. These plates were inoculated at 37 °C for 2 days.</p>
+
<td><em>trpC2</em> 1.3&nbsp;kbp deletion <em>5'-yhfE-yhfF-3'-gltT</em></td>
−
 
+
</tr>
 +
<tr>
 +
<td>iGEM2</td>
 +
<td>Suppressor 2 10 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM3</td>
 +
<td>Suppressor 3 10 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM4</td>
 +
<td>Suppressor 4 10 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM5</td>
 +
<td>Suppressor 5 10 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM6</td>
 +
<td>Suppressor 1-1 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM7</td>
 +
<td>Suppressor 1-2 30 mM</td>
 +
<td><em>trpC2</em> 1.3&nbsp;kbp deletion <em>5'-yhfE-yhfF-3'-gltT gltP</em>(G232A)</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM8</td>
 +
<td>Suppressor 2-1 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM9</td>
 +
<td>Suppressor 2-2 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM10</td>
 +
<td>Suppressor 3-1 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM11</td>
 +
<td>Suppressor 3-2 30 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM12</td>
 +
<td>Suppressor 1-1 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM13</td>
 +
<td>Suppressor 1-2 40 mM</td>
 +
<td><em>trpC2</em> 1.3&nbsp;kbp deletion <em>5'-yhfE-yhfF-3'-gltT</em> 11&nbsp;bp deletion in <em>gltP</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM14</td>
 +
<td>Suppressor 2-1 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM15</td>
 +
<td>Suppressor 2-2 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM16</td>
 +
<td>Suppressor 3-1 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td>iGEM17</td>
 +
<td>Suppressor 3-2 40 mM</td>
 +
<td><em>trpC2</em> not determined</td>
 +
</tr>
 +
<tr>
 +
<td> BP233</td>
 +
<td></td>
 +
<td><em> trpC2</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td> BP234</td>
 +
<td></td>
 +
<td><em>trpC2</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>BP235</td>
 +
<td></td>
 +
<td><em> trpC2</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>BP236</td>
 +
<td></td>
 +
<td> <em> trpC2 amyE::(Palf4-gltT-lacZ aphA3)</em> </td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>BP237</td>
 +
<td></td>
 +
<td> <em> trpC2</em> &Delta;<em>gltT::spc amyE::(Palf4-gltT-lacZ aphA3)</em> </td>
 +
</tr>
 +
<tr>
 +
<td>iGEM18</td>
 +
<td></td>
 +
<td><em>amyE::(PtrpE-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM19</td>
 +
<td></td>
 +
<td><em>trpC2 amyE::(PtrpE-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM20</td>
 +
<td>SP1_GP342</td>
 +
<td> <em> amyE::(PgltA-lacZ aphA3)</em> </td>
 +
</tr>
 +
<tr>
 +
<td>iGEM21</td>
 +
<td>SP1_GP650</td>
 +
<td> <em> amyE::(PgltA-lacZ aphA3) gltC::TnIO spc </em> </td>
 +
</tr>
 +
<tr>
 +
<td>iGEM22 </td>
 +
<td>168::BFP</td>
 +
<td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM23 </td>
 +
<td>168::GFP</td>
 +
<td> <em>trpC2 amyE::(GFP-lacZ aphA3) </em></td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>iGEM24</td>
 +
<td>168::mOrange</td>
 +
<td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> &Delta;<em>gltT::spc </em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM25</td>
 +
<td>168::mCherry</td>
 +
<td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM26</td>
 +
<td>BP233::BFP</td>
 +
<td> <em>trpC2 amyE::(BFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM27</td>
 +
<td>BP233::GFP</td>
 +
<td> <em>trpC2 amyE::(GFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM28</td>
 +
<td>BP233::mOrange</td>
 +
<td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> &Delta;<em>gltT::spc </em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM29</td>
 +
<td>BP233::mCherry</td>
 +
<td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM30</td>
 +
<td>Spden::BFP</td>
 +
<td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat </em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM31</td>
 +
<td>Spden::GFP </td>
 +
<td> <em> trpC2 amyE::(GFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM32</td>
 +
<td>Spden::mOrange</td>
 +
<td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM33</td>
 +
<td>Spden::mCherry</td>
 +
<td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM34</td>
 +
<td>BP235::BFP</td>
 +
<td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em> </td>
 +
</tr>
 +
<tr>
 +
<td>iGEM35</td>
 +
<td>BP235::GFP</td>
 +
<td> <em> trpC2 amyE::(GFP-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM36</td>
 +
<td>BP235::mOrange</td>
 +
<td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> &Delta;<em>gltT::spc</em> &Delta;<em>gltP::cat</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM37</td>
 +
<td>SP1::Gat</td>
 +
<td><em>amyE::(gat-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM38</td>
 +
<td>SP1::AroA*</td>
 +
<td><em>amyE::(aroA*-lacZ aphA3)</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM39</td>
 +
<td>SP1::Gat::&Delta;<em>gltT</em></td>
 +
<td><em>amyE::(gat-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM40</td>
 +
<td>SP1::AroA*::&Delta;<em>gltT</em></td>
 +
<td><em>amyE::(aroA*-lacZ aphA3)</em> &Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
<tr>
 +
<td>iGEM41</td>
 +
<td>SP1::&Delta;<em>gltT</em></td>
 +
<td>&Delta;<em>gltT::spc</em></td>
 +
</tr>
 +
</table>
 
</div>
 
</div>
 +
  </div>
 
</div>
 
</div>
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−
        <p class="notebook-head_date">04.05.18</p>
 
−
    </div>
 
−
    <div class="notebook-content">
 
−
        <p>The growth of the evolved suppressor mutants of the strains 168, BP233 and BP235 on 10 mM and 0 mM glyphosate CS-glucose agar plates was documented.</p>
 
−
        <p>A growth test of 168, BP233 and BP237 on CS-Glucose agar plates containing 0 mM and 10 mM glyphosate.</p>
 
−
    </div>
 
 
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Latest revision as of 11:25, 5 October 2018

Our Labnotes:

In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months. Beyond this section, our strain list is shown.

Project:

Month:

STRAIN LIST

Name Name after isolation
Genotype
168 trpC2
SP1 wildtype
iGEM1 Suppressor 1 10 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT
iGEM2 Suppressor 2 10 mM trpC2 not determined
iGEM3 Suppressor 3 10 mM trpC2 not determined
iGEM4 Suppressor 4 10 mM trpC2 not determined
iGEM5 Suppressor 5 10 mM trpC2 not determined
iGEM6 Suppressor 1-1 30 mM trpC2 not determined
iGEM7 Suppressor 1-2 30 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT gltP(G232A)
iGEM8 Suppressor 2-1 30 mM trpC2 not determined
iGEM9 Suppressor 2-2 30 mM trpC2 not determined
iGEM10 Suppressor 3-1 30 mM trpC2 not determined
iGEM11 Suppressor 3-2 30 mM trpC2 not determined
iGEM12 Suppressor 1-1 40 mM trpC2 not determined
iGEM13 Suppressor 1-2 40 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT 11 bp deletion in gltP
iGEM14 Suppressor 2-1 40 mM trpC2 not determined
iGEM15 Suppressor 2-2 40 mM trpC2 not determined
iGEM16 Suppressor 3-1 40 mM trpC2 not determined
iGEM17 Suppressor 3-2 40 mM trpC2 not determined
BP233 trpC2 ΔgltT::spc
BP234 trpC2 ΔgltP::cat
BP235 trpC2 ΔgltT::spc ΔgltP::cat
BP236 trpC2 amyE::(Palf4-gltT-lacZ aphA3)
BP237 trpC2 ΔgltT::spc amyE::(Palf4-gltT-lacZ aphA3)
iGEM18 amyE::(PtrpE-lacZ aphA3)
iGEM19 trpC2 amyE::(PtrpE-lacZ aphA3)
iGEM20 SP1_GP342 amyE::(PgltA-lacZ aphA3)
iGEM21 SP1_GP650 amyE::(PgltA-lacZ aphA3) gltC::TnIO spc
iGEM22 168::BFP trpC2 amyE::(BFP-lacZ aphA3)
iGEM23 168::GFP trpC2 amyE::(GFP-lacZ aphA3)
iGEM24 168::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc
iGEM25 168::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc
iGEM26 BP233::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc
iGEM27 BP233::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc
iGEM28 BP233::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc
iGEM29 BP233::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc
iGEM30 Spden::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM31 Spden::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM32 Spden::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM33 Spden::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM34 BP235::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM35 BP235::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM36 BP235::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM37 SP1::Gat amyE::(gat-lacZ aphA3)
iGEM38 SP1::AroA* amyE::(aroA*-lacZ aphA3)
iGEM39 SP1::Gat::ΔgltT amyE::(gat-lacZ aphA3) ΔgltT::spc
iGEM40 SP1::AroA*::ΔgltT amyE::(aroA*-lacZ aphA3) ΔgltT::spc
iGEM41 SP1::ΔgltT ΔgltT::spc