Difference between revisions of "Team:Goettingen/Notebook"
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| − | <p> | + | <p>In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months. Beyond this section, our strain list is shown.</p> |
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| + | |||
| + | |||
| + | <div class="article_table"> | ||
| + | <p><strong>STRAIN LIST</strong></p> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>Name</th> | ||
| + | <th>Name after isolation</th> | ||
| + | <th> | ||
| + | <center>Genotype</center> | ||
| + | </th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>168</td> | ||
| + | <td></td> | ||
| + | <td><em>trpC2</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>SP1</td> | ||
| + | <td></td> | ||
| + | <td>wildtype</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM1</td> | ||
| + | <td>Suppressor 1 10 mM</td> | ||
| + | <td><em>trpC2</em> 1.3 kbp deletion <em>5'-yhfE-yhfF-3'-gltT</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM2</td> | ||
| + | <td>Suppressor 2 10 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM3</td> | ||
| + | <td>Suppressor 3 10 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM4</td> | ||
| + | <td>Suppressor 4 10 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM5</td> | ||
| + | <td>Suppressor 5 10 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM6</td> | ||
| + | <td>Suppressor 1-1 30 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM7</td> | ||
| + | <td>Suppressor 1-2 30 mM</td> | ||
| + | <td><em>trpC2</em> 1.3 kbp deletion <em>5'-yhfE-yhfF-3'-gltT gltP</em>(G232A)</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM8</td> | ||
| + | <td>Suppressor 2-1 30 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM9</td> | ||
| + | <td>Suppressor 2-2 30 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM10</td> | ||
| + | <td>Suppressor 3-1 30 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM11</td> | ||
| + | <td>Suppressor 3-2 30 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM12</td> | ||
| + | <td>Suppressor 1-1 40 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM13</td> | ||
| + | <td>Suppressor 1-2 40 mM</td> | ||
| + | <td><em>trpC2</em> 1.3 kbp deletion <em>5'-yhfE-yhfF-3'-gltT</em> 11 bp deletion in <em>gltP</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM14</td> | ||
| + | <td>Suppressor 2-1 40 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM15</td> | ||
| + | <td>Suppressor 2-2 40 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM16</td> | ||
| + | <td>Suppressor 3-1 40 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM17</td> | ||
| + | <td>Suppressor 3-2 40 mM</td> | ||
| + | <td><em>trpC2</em> not determined</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> BP233</td> | ||
| + | <td></td> | ||
| + | <td><em> trpC2</em> Δ<em>gltT::spc</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td> BP234</td> | ||
| + | <td></td> | ||
| + | <td><em>trpC2</em> Δ<em>gltP::cat</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>BP235</td> | ||
| + | <td></td> | ||
| + | <td><em> trpC2</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>BP236</td> | ||
| + | <td></td> | ||
| + | <td> <em> trpC2 amyE::(Palf4-gltT-lacZ aphA3)</em> </td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>BP237</td> | ||
| + | <td></td> | ||
| + | <td> <em> trpC2</em> Δ<em>gltT::spc amyE::(Palf4-gltT-lacZ aphA3)</em> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM18</td> | ||
| + | <td></td> | ||
| + | <td><em>amyE::(PtrpE-lacZ aphA3)</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM19</td> | ||
| + | <td></td> | ||
| + | <td><em>trpC2 amyE::(PtrpE-lacZ aphA3)</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM20</td> | ||
| + | <td>SP1_GP342</td> | ||
| + | <td> <em> amyE::(PgltA-lacZ aphA3)</em> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM21</td> | ||
| + | <td>SP1_GP650</td> | ||
| + | <td> <em> amyE::(PgltA-lacZ aphA3) gltC::TnIO spc </em> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM22 </td> | ||
| + | <td>168::BFP</td> | ||
| + | <td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM23 </td> | ||
| + | <td>168::GFP</td> | ||
| + | <td> <em>trpC2 amyE::(GFP-lacZ aphA3) </em></td> | ||
| + | </tr> | ||
| + | |||
| + | <tr> | ||
| + | <td>iGEM24</td> | ||
| + | <td>168::mOrange</td> | ||
| + | <td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> Δ<em>gltT::spc </em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM25</td> | ||
| + | <td>168::mCherry</td> | ||
| + | <td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM26</td> | ||
| + | <td>BP233::BFP</td> | ||
| + | <td> <em>trpC2 amyE::(BFP-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM27</td> | ||
| + | <td>BP233::GFP</td> | ||
| + | <td> <em>trpC2 amyE::(GFP-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM28</td> | ||
| + | <td>BP233::mOrange</td> | ||
| + | <td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> Δ<em>gltT::spc </em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM29</td> | ||
| + | <td>BP233::mCherry</td> | ||
| + | <td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM30</td> | ||
| + | <td>Spden::BFP</td> | ||
| + | <td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat </em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM31</td> | ||
| + | <td>Spden::GFP </td> | ||
| + | <td> <em> trpC2 amyE::(GFP-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM32</td> | ||
| + | <td>Spden::mOrange</td> | ||
| + | <td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM33</td> | ||
| + | <td>Spden::mCherry</td> | ||
| + | <td> <em> trpC2 amyE::(mCherry-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM34</td> | ||
| + | <td>BP235::BFP</td> | ||
| + | <td> <em> trpC2 amyE::(BFP-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM35</td> | ||
| + | <td>BP235::GFP</td> | ||
| + | <td> <em> trpC2 amyE::(GFP-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM36</td> | ||
| + | <td>BP235::mOrange</td> | ||
| + | <td> <em> trpC2 amyE::(mOrange-lacZ aphA3)</em> Δ<em>gltT::spc</em> Δ<em>gltP::cat</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM37</td> | ||
| + | <td>SP1::Gat</td> | ||
| + | <td><em>amyE::(gat-lacZ aphA3)</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM38</td> | ||
| + | <td>SP1::AroA*</td> | ||
| + | <td><em>amyE::(aroA*-lacZ aphA3)</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM39</td> | ||
| + | <td>SP1::Gat::Δ<em>gltT</em></td> | ||
| + | <td><em>amyE::(gat-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM40</td> | ||
| + | <td>SP1::AroA*::Δ<em>gltT</em></td> | ||
| + | <td><em>amyE::(aroA*-lacZ aphA3)</em> Δ<em>gltT::spc</em></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>iGEM41</td> | ||
| + | <td>SP1::Δ<em>gltT</em></td> | ||
| + | <td>Δ<em>gltT::spc</em></td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Latest revision as of 11:25, 5 October 2018
Team Göttingen
iGEM 2018
Glyphosate on my plate?
| E-mail: | igem2018@gwdg.de |
|---|---|
| Adress: | c/o iGEM 2018 |
| Department of General Microbiology | |
| Grisebachstraße 8 | |
| 37077 Göttingen | |
| Germany |
We are especially grateful to our numerous sponsors on gofundme, who supported our project and made it possible.
Every public donor, who helped us to achieve our goals got a special place on our bacterial wall of fame and a handdrawn microbe to show our appreciation. A link to that wall can be found here:
Our Labnotes:
STRAIN LIST
| Name | Name after isolation |
|
|---|---|---|
| 168 | trpC2 | |
| SP1 | wildtype | |
| iGEM1 | Suppressor 1 10 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT |
| iGEM2 | Suppressor 2 10 mM | trpC2 not determined |
| iGEM3 | Suppressor 3 10 mM | trpC2 not determined |
| iGEM4 | Suppressor 4 10 mM | trpC2 not determined |
| iGEM5 | Suppressor 5 10 mM | trpC2 not determined |
| iGEM6 | Suppressor 1-1 30 mM | trpC2 not determined |
| iGEM7 | Suppressor 1-2 30 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT gltP(G232A) |
| iGEM8 | Suppressor 2-1 30 mM | trpC2 not determined |
| iGEM9 | Suppressor 2-2 30 mM | trpC2 not determined |
| iGEM10 | Suppressor 3-1 30 mM | trpC2 not determined |
| iGEM11 | Suppressor 3-2 30 mM | trpC2 not determined |
| iGEM12 | Suppressor 1-1 40 mM | trpC2 not determined |
| iGEM13 | Suppressor 1-2 40 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT 11 bp deletion in gltP |
| iGEM14 | Suppressor 2-1 40 mM | trpC2 not determined |
| iGEM15 | Suppressor 2-2 40 mM | trpC2 not determined |
| iGEM16 | Suppressor 3-1 40 mM | trpC2 not determined |
| iGEM17 | Suppressor 3-2 40 mM | trpC2 not determined |
| BP233 | trpC2 ΔgltT::spc | |
| BP234 | trpC2 ΔgltP::cat | |
| BP235 | trpC2 ΔgltT::spc ΔgltP::cat | |
| BP236 | trpC2 amyE::(Palf4-gltT-lacZ aphA3) | |
| BP237 | trpC2 ΔgltT::spc amyE::(Palf4-gltT-lacZ aphA3) | |
| iGEM18 | amyE::(PtrpE-lacZ aphA3) | |
| iGEM19 | trpC2 amyE::(PtrpE-lacZ aphA3) | |
| iGEM20 | SP1_GP342 | amyE::(PgltA-lacZ aphA3) |
| iGEM21 | SP1_GP650 | amyE::(PgltA-lacZ aphA3) gltC::TnIO spc |
| iGEM22 | 168::BFP | trpC2 amyE::(BFP-lacZ aphA3) |
| iGEM23 | 168::GFP | trpC2 amyE::(GFP-lacZ aphA3) |
| iGEM24 | 168::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc |
| iGEM25 | 168::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc |
| iGEM26 | BP233::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc |
| iGEM27 | BP233::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc |
| iGEM28 | BP233::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc |
| iGEM29 | BP233::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc |
| iGEM30 | Spden::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM31 | Spden::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM32 | Spden::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM33 | Spden::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM34 | BP235::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM35 | BP235::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM36 | BP235::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM37 | SP1::Gat | amyE::(gat-lacZ aphA3) |
| iGEM38 | SP1::AroA* | amyE::(aroA*-lacZ aphA3) |
| iGEM39 | SP1::Gat::ΔgltT | amyE::(gat-lacZ aphA3) ΔgltT::spc |
| iGEM40 | SP1::AroA*::ΔgltT | amyE::(aroA*-lacZ aphA3) ΔgltT::spc |
| iGEM41 | SP1::ΔgltT | ΔgltT::spc |