Difference between revisions of "Team:Goettingen/Notebook"

 
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     <p>In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months.</p>
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     <p>In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months. Beyond this section, our strain list is shown.</p>
 
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<h2><center>Strain list</center><h2>
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<p><strong>STRAIN LIST</strong></p>
 
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Latest revision as of 11:25, 5 October 2018

Our Labnotes:

In this section, you can see our lab notes. Our topic is divided into four subtopics which are the following ones. You can choose between "Fluorophores", "Biobricks", "Evolution", and "Reporter-Systems" and the respective months. Beyond this section, our strain list is shown.

Project:

Month:

STRAIN LIST

Name Name after isolation
Genotype
168 trpC2
SP1 wildtype
iGEM1 Suppressor 1 10 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT
iGEM2 Suppressor 2 10 mM trpC2 not determined
iGEM3 Suppressor 3 10 mM trpC2 not determined
iGEM4 Suppressor 4 10 mM trpC2 not determined
iGEM5 Suppressor 5 10 mM trpC2 not determined
iGEM6 Suppressor 1-1 30 mM trpC2 not determined
iGEM7 Suppressor 1-2 30 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT gltP(G232A)
iGEM8 Suppressor 2-1 30 mM trpC2 not determined
iGEM9 Suppressor 2-2 30 mM trpC2 not determined
iGEM10 Suppressor 3-1 30 mM trpC2 not determined
iGEM11 Suppressor 3-2 30 mM trpC2 not determined
iGEM12 Suppressor 1-1 40 mM trpC2 not determined
iGEM13 Suppressor 1-2 40 mM trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT 11 bp deletion in gltP
iGEM14 Suppressor 2-1 40 mM trpC2 not determined
iGEM15 Suppressor 2-2 40 mM trpC2 not determined
iGEM16 Suppressor 3-1 40 mM trpC2 not determined
iGEM17 Suppressor 3-2 40 mM trpC2 not determined
BP233 trpC2 ΔgltT::spc
BP234 trpC2 ΔgltP::cat
BP235 trpC2 ΔgltT::spc ΔgltP::cat
BP236 trpC2 amyE::(Palf4-gltT-lacZ aphA3)
BP237 trpC2 ΔgltT::spc amyE::(Palf4-gltT-lacZ aphA3)
iGEM18 amyE::(PtrpE-lacZ aphA3)
iGEM19 trpC2 amyE::(PtrpE-lacZ aphA3)
iGEM20 SP1_GP342 amyE::(PgltA-lacZ aphA3)
iGEM21 SP1_GP650 amyE::(PgltA-lacZ aphA3) gltC::TnIO spc
iGEM22 168::BFP trpC2 amyE::(BFP-lacZ aphA3)
iGEM23 168::GFP trpC2 amyE::(GFP-lacZ aphA3)
iGEM24 168::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc
iGEM25 168::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc
iGEM26 BP233::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc
iGEM27 BP233::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc
iGEM28 BP233::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc
iGEM29 BP233::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc
iGEM30 Spden::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM31 Spden::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM32 Spden::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM33 Spden::mCherry trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM34 BP235::BFP trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM35 BP235::GFP trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM36 BP235::mOrange trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat
iGEM37 SP1::Gat amyE::(gat-lacZ aphA3)
iGEM38 SP1::AroA* amyE::(aroA*-lacZ aphA3)
iGEM39 SP1::Gat::ΔgltT amyE::(gat-lacZ aphA3) ΔgltT::spc
iGEM40 SP1::AroA*::ΔgltT amyE::(aroA*-lacZ aphA3) ΔgltT::spc
iGEM41 SP1::ΔgltT ΔgltT::spc