Difference between revisions of "Team:Goettingen/Parts"
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<p>Basic parts are functional DNA units that cannot be divided into smaller parts. A construct of multiple basic parts is called a composite part. Here, the functionality of a basic part was increased through the implementation of different functional sites. The parts of our team are listed and shortly described in the following table: </p> | <p>Basic parts are functional DNA units that cannot be divided into smaller parts. A construct of multiple basic parts is called a composite part. Here, the functionality of a basic part was increased through the implementation of different functional sites. The parts of our team are listed and shortly described in the following table: </p> | ||
</div> | </div> | ||
| − | |||
<div class="notebook-table article_table"> | <div class="notebook-table article_table"> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586000" target="_blank">BBa_K2586000</a>/<i>P<sub>alf4</sub>< | + | <td><a href="http://parts.igem.org/Part:BBa_K2586000" target="_blank">BBa_K2586000</a>/<i>P<sub>alf4</sub></i></td> |
<td>Promoter for <em>gltT</em> expression </td> | <td>Promoter for <em>gltT</em> expression </td> | ||
<td>Promoter</td> | <td>Promoter</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586001" target="_blank">BBa_K2586001/<i>gltT< | + | <td><a href="http://parts.igem.org/Part:BBa_K2586001" target="_blank">BBa_K2586001</a>/<i>gltT</i></td> |
<td>Uptake of glutamate from the environment </td> | <td>Uptake of glutamate from the environment </td> | ||
<td>Coding</td> | <td>Coding</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586002" target="_blank">BBa_K2586002/<i>gltP< | + | <td><a href="http://parts.igem.org/Part:BBa_K2586002" target="_blank">BBa_K2586002</a>/<i>gltP</i></td> |
<td>Uptake of glutamate from the environment </td> | <td>Uptake of glutamate from the environment </td> | ||
<td>Coding</td> | <td>Coding</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586003" target="_blank">BBa_K2586003/<i>aroE< | + | <td><a href="http://parts.igem.org/Part:BBa_K2586003" target="_blank">BBa_K2586003</a>/<i>aroE</i></td> |
<td>Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate</td> | <td>Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 52: | Line 51: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586005" target="_blank">BBa_K2586005/<i>P<sub>trpP</sub>< | + | <td><a href="http://parts.igem.org/Part:BBa_K2586005" target="_blank">BBa_K2586005</a>/<i>P<sub>trpP</sub></i></td> |
| − | <td>Promoter for <em>P<sub>trpP</sub> in <em>Bacillus subtilis<em></td> | + | <td>Promoter for <em>P<sub>trpP</sub></em> in <em>Bacillus subtilis</em></td> |
<td>Promoter</td> | <td>Promoter</td> | ||
<td>470</td> | <td>470</td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586007" target="_blank">BBa_K2586007/<i>aroA< | + | <td><a href="http://parts.igem.org/Part:BBa_K2586007" target="_blank">BBa_K2586007</a>/<i>aroA</i></td> |
<td>Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate</td> | <td>Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
| Line 64: | Line 63: | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586008" target="_blank">BBa_K2586008/RBS</td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2586008" target="_blank">BBa_K2586008</a>/RBS</td> |
<td>Ribosome binding site</td> | <td>Ribosome binding site</td> | ||
<td>Regulatory</td> | <td>Regulatory</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586010" target="_blank">BBa_K2586010/<i>P<sub>alf4</sub>< | + | <td><a href="http://parts.igem.org/Part:BBa_K2586010" target="_blank">BBa_K2586010</a>/<i>P<sub>alf4</sub></i>-RBS</td> |
<td>Promoter connected to RBS</td> | <td>Promoter connected to RBS</td> | ||
<td>Composite</td> | <td>Composite</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586019" target="_blank">BBa_K2586019/<i>gat</i></td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2586019" target="_blank">BBa_K2586019</a>/<i>gat</i></td> |
<td>Glyphosate N-acetyl-transferase</td> | <td>Glyphosate N-acetyl-transferase</td> | ||
<td>Coding</td> | <td>Coding</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586020" target="_blank">BBa_K2586020/<i>aroA*< | + | <td><a href="http://parts.igem.org/Part:BBa_K2586020" target="_blank">BBa_K2586020</a>/<i>aroA*</i></td> |
<td>Mutated <em>aroA</em></td> | <td>Mutated <em>aroA</em></td> | ||
<td>Coding</td> | <td>Coding</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part:BBa_K2586021" target="_blank">BBa_K2586021/RBS-<i>gltT</i></td> | + | <td><a href="http://parts.igem.org/Part:BBa_K2586021" target="_blank">BBa_K2586021</a>/RBS-<i>gltT</i></td> |
<td>RBS connected to <em>gltT</em></td> | <td>RBS connected to <em>gltT</em></td> | ||
<td>Composite</td> | <td>Composite</td> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
| − | |||
</div> | </div> | ||
| + | |||
<p>Furthermore, we characterized an existing part. We chose <a href="http://parts.igem.org/Part:BBa_E2050" target="_blank">BBa_E2050 (mERP)</a>, wich we transformed into different <em>Bacillus</em> strains. Because the plasmid pSB1C3 does not contain an origin of replication for Bacillus, we cloned the fluorophore into the plasmid pAC7 and transformed it into competent DH5α. The fluorophore was additionally coupled to a self-made promoter, which is characterized by a perfect consensus sequence and perfect RBS for <em>Bacillus</em>. Further information can be found on the Registry. Additionally, we used the strains for further experiments (check out the Results section). </p> | <p>Furthermore, we characterized an existing part. We chose <a href="http://parts.igem.org/Part:BBa_E2050" target="_blank">BBa_E2050 (mERP)</a>, wich we transformed into different <em>Bacillus</em> strains. Because the plasmid pSB1C3 does not contain an origin of replication for Bacillus, we cloned the fluorophore into the plasmid pAC7 and transformed it into competent DH5α. The fluorophore was additionally coupled to a self-made promoter, which is characterized by a perfect consensus sequence and perfect RBS for <em>Bacillus</em>. Further information can be found on the Registry. Additionally, we used the strains for further experiments (check out the Results section). </p> | ||
Revision as of 11:27, 5 October 2018
Team Göttingen
iGEM 2018
Glyphosate on my plate?
| E-mail: | igem2018@gwdg.de |
|---|---|
| Adress: | c/o iGEM 2018 |
| Department of General Microbiology | |
| Grisebachstraße 8 | |
| 37077 Göttingen | |
| Germany |
We are especially grateful to our numerous sponsors on gofundme, who supported our project and made it possible.
Every public donor, who helped us to achieve our goals got a special place on our bacterial wall of fame and a handdrawn microbe to show our appreciation. A link to that wall can be found here:
Parts
Connecting global research
Our contribution to a huge parts collection.
The concept of iGEM is based on the exchange of internationally provided DNA sequences. These sequences are called Biobricks and are collected in a big database, which grows steadily as iGEM progresses. In this way, teams all over the globe can benefit from the part collection and improve the work that was done previously by other teams, to drive the research further.
Basic parts and composite parts
Basic parts are functional DNA units that cannot be divided into smaller parts. A construct of multiple basic parts is called a composite part. Here, the functionality of a basic part was increased through the implementation of different functional sites. The parts of our team are listed and shortly described in the following table:
| Part Number and Name | Short Description | Type | Length [bp] |
|---|---|---|---|
| BBa_K2586000/Palf4 | Promoter for gltT expression | Promoter | 30 |
| BBa_K2586001/gltT | Uptake of glutamate from the environment | Coding | 1290 |
| BBa_K2586002/gltP | Uptake of glutamate from the environment | Coding | 1245 |
| BBa_K2586003/aroE | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | Coding | 1287 |
| BBa_K2586005/PtrpP | Promoter for PtrpP in Bacillus subtilis | Promoter | 470 |
| BBa_K2586007/aroA | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | Coding | 1290 |
| BBa_K2586008/RBS | Ribosome binding site | Regulatory | 13 |
| BBa_K2586010/Palf4-RBS | Promoter connected to RBS | Composite | 94 |
| BBa_K2586019/gat | Glyphosate N-acetyl-transferase | Coding | 480 |
| BBa_K2586020/aroA* | Mutated aroA | Coding | 480 |
| BBa_K2586021/RBS-gltT | RBS connected to gltT | Composite | 1355 |
Furthermore, we characterized an existing part. We chose BBa_E2050 (mERP), wich we transformed into different Bacillus strains. Because the plasmid pSB1C3 does not contain an origin of replication for Bacillus, we cloned the fluorophore into the plasmid pAC7 and transformed it into competent DH5α. The fluorophore was additionally coupled to a self-made promoter, which is characterized by a perfect consensus sequence and perfect RBS for Bacillus. Further information can be found on the Registry. Additionally, we used the strains for further experiments (check out the Results section).