Difference between revisions of "Team:Nanjing-China/Background"
| Line 84: | Line 84: | ||
<div align="center"> | <div align="center"> | ||
<div id="HOME"> | <div id="HOME"> | ||
| − | |||
| − | |||
<div class="sub"> | <div class="sub"> | ||
<ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li></ul></div> | <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li></ul></div> | ||
| Line 94: | Line 92: | ||
<li><a href="#principle">principle</a></li> | <li><a href="#principle">principle</a></li> | ||
<li><a href="#whole">whole</a></li></ul> | <li><a href="#whole">whole</a></li></ul> | ||
| + | <div class="sub"> | ||
| + | <ul> <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li></div> | ||
| + | <div class="sub"> | ||
| + | <ul> <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li></div> | ||
| + | <div class="sub"> | ||
| + | <ul> <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li></div> | ||
| + | <div class="sub"> | ||
| + | <ul> <li><a href="https://2018.igem.org/Team:Nanjing-China/Hardware">Hardware</a></li></div> | ||
| + | <div class="sub"> | ||
| + | <ul> <li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></li></div> | ||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
<div class="container" align="center"> | <div class="container" align="center"> | ||
| − | + | <div id="menu"> | |
| − | + | <ul> | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China"> | + | <li><a href="https://2018.igem.org/Team:Nanjing-China">People</a> |
| + | <ul> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Team">Team</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Members">Members</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Attributions">Attributions</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/For_Judges">For_Judges</a></li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">PROJECT</a> | ||
<ul> | <ul> | ||
| − | |||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Background">Background</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Design">Design</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Demonstrate">Demonstrate</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Hardware">Hardware</a></li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/InterLab">InterLab</a></li> | ||
</ul> | </ul> | ||
| − | + | </li> | |
<li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Parts">PARTS</a> | ||
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Basic_Part">Basic_Part</a></li> | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Composite_Part">Composite</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Composite_Part">Composite</a></li> | ||
| − | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Improve">Improve</a></li> | |
</ul> | </ul> | ||
</li> | </li> | ||
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/ | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Model">Model</a> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</li> | </li> | ||
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/ | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices">Practices</a> |
<ul> | <ul> | ||
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/ | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices"><font size="-1">Human_Practices</font></a></li> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li> | <li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></li> | ||
| − | + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></li> | |
| − | <li><a href="https://2018.igem.org/Team:Nanjing-China/ | + | |
| − | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
| + | <li><a href="https://2018.igem.org/Team:Nanjing-China/Notebook">Notebook</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| − | <div class="header"><img src="https://static.igem.org/mediawiki/2018/ | + | <div class="header"><img src="https://static.igem.org/mediawiki/2018/4/40/T--Nanjing-China--title-4.png" width="100%" > |
</div> | </div> | ||
<div class="contain"> | <div class="contain"> | ||
| Line 293: | Line 290: | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="f-b"><img src="https://static.igem.org/mediawiki/2018/ | + | <div class="f-b"><img src="https://static.igem.org/mediawiki/2018/5/58/T--Nanjing-China--footer-4.png" width="100%" /></div> |
</div> | </div> | ||
</div> | </div> | ||
</body> | </body> | ||
</html> | </html> | ||
Revision as of 10:49, 6 October 2018
The definition of nitrogen fixation
•What is nitrogen fixation?
Nitrogen → ammonia (NH3) or other molecules available to living organisms.
What is nitrogen fixation for?
-global food supply
-reduce the use of chemical nitrogen fertilizers
Nitrogen fixation is essential for life.
The methods of nitrogem fixation
Nitrogen cycle
•Nitrogen fixation:
—N2 → plants by bacteria
•Nitrification:
—ammonium → nitrite → nitrate
—Absorbed by plants
•Denitrification:
—Release N to atmosphere
Haber-Bosch process
N2 + 3H2 → 2NH3(ΔH° = −91.8 kJ)
High temperature
High pressure
Need too much energy
Biological nitrogen fixation
Mild reaction conditions
Relatively inexpensive
High efficiency
Environmentally friendly
The choose of nitrogenase
Molybdenum
(MoFe)-dependent
Vanadium
(VFe)-dependent
Iron-only
(FeFe)-dependent
MoFe has been studied extensively
Nitrogen Fixation Gene Cluster
The minimal nif genes required for nitrogen fixation.
General principle of biological nitrogen fixation
| 01 Hydrolysis of ATP | 03 MoFe protein forming complexes with low-potential donor | |
 Chemical energy (ATP)→Solar energy |
||
| 02 Electron transfer | 04 Reduce N2 to NH3 |
| 01 Receive light signals | 03 MoFe protein forming complexes with low-potential donor | |
 |
||
| 02 Electron transfer | 04 Reduce N2 to NH3 |
The characteristics of whole-cell
The property and advantage of whole-cells
•Fast proliferation to enable large-scale application
•Good portability to permit on-site monitoring
•Inexpensive and easy to preserve
• Intracellular cascade reactions to amplify signals
• Excellent specificity, sensitivity and stability
Address
Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046
