Difference between revisions of "Team:CCU Taiwan/Safety"
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<p class="description">Genetically modified organisms escaping from lab is a serious problem since it will bring unpredictable impacts to our ecosystem. In our part design and production line, we were very conscious of biosafety. The yeast will pass the following process to achieve complete destruction. We also dealt cautiously with waste produced from the experiment and participated in safety training to ensure everyone conducting experiments had a minimal probability to cause biohazard pollution. | <p class="description">Genetically modified organisms escaping from lab is a serious problem since it will bring unpredictable impacts to our ecosystem. In our part design and production line, we were very conscious of biosafety. The yeast will pass the following process to achieve complete destruction. We also dealt cautiously with waste produced from the experiment and participated in safety training to ensure everyone conducting experiments had a minimal probability to cause biohazard pollution. | ||
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Revision as of 13:26, 9 October 2018
Safety
Genetically modified organisms escaping from lab is a serious problem since it will bring unpredictable impacts to our ecosystem. In our part design and production line, we were very conscious of biosafety. The yeast will pass the following process to achieve complete destruction. We also dealt cautiously with waste produced from the experiment and participated in safety training to ensure everyone conducting experiments had a minimal probability to cause biohazard pollution.
Filtering system
The average size of the P. pastoris used is about 4–6 μm [1], and the filter we used is 33 kDa, which is equivalent to the size of tens of nanometers [2], more than sufficient to trap all the yeast.
Heating system
According to the literature [3], heating at 70 °C for 30 minutes is sufficient to kill the cells. We used a temperature of 110 ° C for 10 minutes to kill any cells that might pass through the filter.
Reference
- 1. Gmeiner, C., Saadati, A., Maresch, D., Krasteva, S., Frank, M., Altmann, F., … Spadiut, O. (2015). Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase. Microbial Cell Factories, 14(1).
doi:10.1186/s12934-014-0183-3 - Bacher, G., Szymanski, W. W., Kaufman, S. L., Zöllner, P., Blaas, D., & Allmaier, G. (2001). Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses.
Journal of Mass Spectrometry, 36(9), 1038–1052.
doi:10.1002/jms.208 - 3. Martínez, D., Menéndez, C., Echemendia, F. M., Hernández, L., Sobrino, A., & Trujillo, L. E. (2015). Kinetics of sucrose hydrolysis by immobilized recombinant Pichia pastoris cells in a batch reactors. J Microb Biochem Technol, 7, 294-6.
- 4. Cregg, J. M., Barringer, K. J., Hessler, A. Y., & Madden, K. R. (1985). Pichia pastoris as a host system for transformations. Molecular and Cellular Biology, 5(12), 3376–3385.
doi:10.1128/mcb.5.12.3376 - 5. Ahmad, M., Hirz, M., Pichler, H., & Schwab, H. (2014). Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Applied Microbiology and Biotechnology, 98(12), 5301–5317.
doi:10.1007/s00253-014-5732-5