Difference between revisions of "Team:Nanjing-China/Results"

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       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Human_Practices"><font size="-1">Human_Practices</font></a></li></ul></div>
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       <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Results">Results</a></ul></li></div>
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Safety">Safety</a></ul></li></div>
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     <li><a href="#cds">CdS</a></li>
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  <li><a href="#nit"><font size="-1">Nitrogen fixation</font></a></li>
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      <ul><li><a href="https://2018.igem.org/Team:Nanjing-China/Collaborations">Collaboration</a></ul></li></div>
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     <li><a href="#overview">Overview</a></li>
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            <li><a href="#language"><font size="-1">Language project</font></a></li>
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            <li><a href="#meet"><font size="-1"> Sharing meeting</font></a></li>
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            <li><a href="#CSU">CSU-China</a></li>
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            <li><a href="#Emoji"><font size="-1">Emoji chanllenge</font></a></li>
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   <li><a href="https://2018.igem.org/Team:Nanjing-China">PEOPLE</a>
 
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      <p>Collaboration has alwayed played a crucial part in human society. So it is with the iGEM competition. Through various meaningful collaborations with teams around the world, we truly experienced the joy of helping and sharing. Meanwhile, it is also a great chance to spread the idea of iGEM to more people.</p>
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    <img src="https://static.igem.org/mediawiki/2018/d/da/T--Nanjing-China--PROJECT-r.jpg" width="50%" /></div>
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      <p>In terms of that, we Nanjing-China host our own meet up as well as enthusiastically respond to collaboration invitations from other teams.</p>
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      <p>list: language project, Nanjing area sharing meeting, helping build a team, Emoji challenge</p>
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<div class="word" id="cds">
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        <h2>Biosynthesis of CdS semiconductor</h2>
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    <img src="https://static.igem.org/mediawiki/2018/a/a7/T--Nanjing-China--TEX-EDX.jpg" width="100%" />
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    <p>TEM-EDX analysis of CdS semiconductor. a) TEM images of biosynthesized CdS semiconductor on the surface of an engineered E. coli cell. b) Elemental analysis using EDX system, the result show that the semiconductor on cell surface is mainly composed of cadmium and sulfide.</p>
 
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     <h2>1.Language project with IIT Madras</h2>
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    <div class="word-2" style="width:60%;" align="left">This year, we contacted team IIT Madras and worked with them to make an educational video on synthetic biology. Not only did we help translate the scripts into Chinese, we also recorded the audio of all the lines. Our effort contributed to their multi-language project with the Mandarin version.<br />
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Later, we received the finished video. Please enjoy the video below:<p>
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<p>Toxicity test was conducted to determine the maximum amount of Cd<sup>2+</sup> that is agreeable for E. coli growth. Compared with the control group that doesn’t contain surface-display gene, our constructed E. coli strain is more sensitive to Cd<sup>2+</sup>, and its growth will be restricted When the Cd<sup>2+</sup> concentration is above 150μM. So we select 100μM as the final Cd<sup>2+</sup> concentration for our further assays.</p>
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     <h2>2.A meet up with iGEM teams in Nanjing</h2>
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    <p>We have effectively communicated with many  teams in various forms. <br />
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    <p>The amount of biosynthesized CdS semiconductor on the E. coli cell surface was measured using inductively coupled plasma mass spectrometry (ICP-MS). These data confirmed the surface-displayed PbrR-mediated biological precipitation of CdS semiconductor on the outer membranes of the cells.</p>
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To share with each other our project and to chase perfection, we invited Professor Haoqian Zhang and all teams in Nanjing to hold a meet up at Nanjing University. During the time spent together, we broadened our horizon by listening to the diverse innovative opinions and gained a lot of joy by sharing stories along the way. Furthermore, through this meeting we deepened our understanding of iGEM and improved experimental design and methods to optimize our own project.  <br />
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After that, we also seized the excellent opportunity offered by the fifth CCiC to demonstrate our project to all teams in China and learn from others. Meanwhile, we were more than glad to see everything going well for all the teams in Nanjing we once communicated with. </p>
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     <p>We performed ultraviolet-visible (UV-vis) spectral measurements to directly determine the optical band gap energy of these CdS semiconductor and the photocatalytic capability for the biological precipitation of CdS semiconductor on the outer membranes of the bacterial cells. The lowest-energy transition of the biosynthesized CdS nanoparticles was detected in the visible region of the solar spectrum (Eg = 2.92 eV, labsorption = 424 nm), confirming the photocatalytic ability of the in situ biosynthesized CdS semiconductor.</p>
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     <div class="word-2" style="width:60%;"><p>This year we also formed close connection with CSU-China, which is a team setting foot on iGEM for the first time. To begin with, we introduced everything about iGEM to them as comprehensively as possible. During their time of building up a real team, we offered timely help and advice feasible to solve their problem through social media. <br />
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     <h2>Light-driven nitrogen fixation in E. coli cells</h2>
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It was indeed a pleasure to offer a hand as we are a more experienced team. Except for that, it surprisingly became a chance for us to learn more novel ideas about what else can be done in iGEM. <br />
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We genuinely hope to hear good news from them in Boston!<br />
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<p>To verify the expression of nitrogenase gene, we conducted Real-time Quantitative PCR(QPCR) to detect the transcription level of nif gene cluster in engineered E. coli, using 16S DNA as an internal reference. The result provided the relative expression level of each nif gene in our constructed E. coli strain. After comparing the result with the ideal expression ratio in Paenibacillus CR1 and model the transcription, we plan to optimize the nif gene cluster by adding promoters or altering the position of genes.<br/>
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     <h2>4.Our Emoji chanllenge!</h2>
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Nitrogenase can not only reduce dinitrogen to ammonia but also reduce ethylene to acetylene. Therefore, we use gas chromatography to detect the amount of acetylene reduced, and indirectly detect its nitrogen fixation activity. On the basis of these results, NH<sub>3</sub> production by our engineered E. coli cell–CdS hybrid system is directly related to the biosynthesized CdS semiconductors as well as illumination and anaerobic conditions.</p>
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    <p>To record the happy moments in our lab and to propagate what a lab is like to the public, we posted a collaboration invitation on the official website to make a collection of Lab emoji. We received some feedbacks and learned that another team Lambert iGEM had shared the similar idea with us. They sent us the picture of a cartoon flask and we also downloaded their app to know more about their thoughts. Both of us had such a whale of a time making labs more appealing to the public.
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Revision as of 11:03, 11 October 2018

Nanjing-China2018

Biosynthesis of CdS semiconductor

TEM-EDX analysis of CdS semiconductor. a) TEM images of biosynthesized CdS semiconductor on the surface of an engineered E. coli cell. b) Elemental analysis using EDX system, the result show that the semiconductor on cell surface is mainly composed of cadmium and sulfide.

Toxicity test was conducted to determine the maximum amount of Cd2+ that is agreeable for E. coli growth. Compared with the control group that doesn’t contain surface-display gene, our constructed E. coli strain is more sensitive to Cd2+, and its growth will be restricted When the Cd2+ concentration is above 150μM. So we select 100μM as the final Cd2+ concentration for our further assays.

The amount of biosynthesized CdS semiconductor on the E. coli cell surface was measured using inductively coupled plasma mass spectrometry (ICP-MS). These data confirmed the surface-displayed PbrR-mediated biological precipitation of CdS semiconductor on the outer membranes of the cells.

We performed ultraviolet-visible (UV-vis) spectral measurements to directly determine the optical band gap energy of these CdS semiconductor and the photocatalytic capability for the biological precipitation of CdS semiconductor on the outer membranes of the bacterial cells. The lowest-energy transition of the biosynthesized CdS nanoparticles was detected in the visible region of the solar spectrum (Eg = 2.92 eV, labsorption = 424 nm), confirming the photocatalytic ability of the in situ biosynthesized CdS semiconductor.

Light-driven nitrogen fixation in E. coli cells

To verify the expression of nitrogenase gene, we conducted Real-time Quantitative PCR(QPCR) to detect the transcription level of nif gene cluster in engineered E. coli, using 16S DNA as an internal reference. The result provided the relative expression level of each nif gene in our constructed E. coli strain. After comparing the result with the ideal expression ratio in Paenibacillus CR1 and model the transcription, we plan to optimize the nif gene cluster by adding promoters or altering the position of genes.
Nitrogenase can not only reduce dinitrogen to ammonia but also reduce ethylene to acetylene. Therefore, we use gas chromatography to detect the amount of acetylene reduced, and indirectly detect its nitrogen fixation activity. On the basis of these results, NH3 production by our engineered E. coli cell–CdS hybrid system is directly related to the biosynthesized CdS semiconductors as well as illumination and anaerobic conditions.

Address

Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046

Email

nanjing_china@163.com

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