Difference between revisions of "Team:Goettingen/Part Collection"

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<h3>★  ALERT! </h3>
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      <h2>Part collection for glyphosate resistance</h2>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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      <p>In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil bacterium <em>B. subtilis</em> is glyphosate resistant. The target of glyphosate is the EPSP synthase, which is encoded by the <em>aroE</em> gene in <em>B. subtilis</em> and by the <em>aroA</em> gene in <em>E. coli</em>. If the target is mutated, like in our part <a href="http://parts.igem.org/Part:BBa_K2586020" target="_blank">BBa_K2586020</a>, the cells are characterized by a high glyphosate resistance. If the cells express the gene encoding the glyphosate-<i>N</i>-acetyl-transferase (GAT), they are also resistant. <b>In conclusion, our collection can serve as a powerful toolbox to genetically engineer organisms for enhancing the resistance towards glyphosate and to improve inactivation or degradation of the weedkiller by microbes.</b></p>
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      <div class="notebook-table article_table">
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        <table>
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                <th>Part Number and Name</th>
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                <th><center>Short Description</center></th>
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                <th>Type</th>
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                <th>Length [bp]</th>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586001" target="_blank">BBa_K2586001</a>/<i>gltT</i></td>
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                <td>Uptake of glutamate or glyphosate from the environment</td>
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                <td>Coding</td>
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                <td>1290</td>
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            </tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586002" target="_blank">BBa_K2586002</a>/<i>gltP</i></td>
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                <td>Uptake of glutamate or glyphosate from the environment </td>
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                <td>Coding</td>
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                <td>1245</td>
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            </tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586003" target="_blank">BBa_K2586003</a>/<i>aroE</i></td>
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                <td>Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate</td>
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                <td>Coding</td>
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                <td>1287</td>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586007" target="_blank">BBa_K2586007</a>/<i>aroA</i></td>
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                <td>Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate</td>
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                <td>Coding</td>
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                <td>1290</td>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586019" target="_blank">BBa_K2586019</a>/<i>gat</i></td>
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                <td>Glyphosate N-acetyl-transferase</td>
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                <td>Coding</td>
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                <td>480</td>
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            </tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586020" target="_blank">BBa_K2586020</a>/<i>aroA*</i></td>
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                <td>Mutated <em>aroA</em></td>
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                <td>Coding</td>
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                <td>480</td>
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<h1> Part Collection </h1>
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
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<h3>Note</h3>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<h3>Best Part Collection Special Prize</h3>
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<p>To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a collection of parts you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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Latest revision as of 13:41, 12 October 2018

Part collection for glyphosate resistance

In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil bacterium B. subtilis is glyphosate resistant. The target of glyphosate is the EPSP synthase, which is encoded by the aroE gene in B. subtilis and by the aroA gene in E. coli. If the target is mutated, like in our part BBa_K2586020, the cells are characterized by a high glyphosate resistance. If the cells express the gene encoding the glyphosate-N-acetyl-transferase (GAT), they are also resistant. In conclusion, our collection can serve as a powerful toolbox to genetically engineer organisms for enhancing the resistance towards glyphosate and to improve inactivation or degradation of the weedkiller by microbes.

Part Number and Name
Short Description
 Type Length [bp]
BBa_K2586001/gltT Uptake of glutamate or glyphosate from the environment Coding 1290
BBa_K2586002/gltP Uptake of glutamate or glyphosate from the environment Coding 1245
BBa_K2586003/aroE Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate Coding 1287
BBa_K2586007/aroA Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate Coding 1290
BBa_K2586019/gat Glyphosate N-acetyl-transferase Coding 480
BBa_K2586020/aroA* Mutated aroA Coding 480