Difference between revisions of "Team:WPI Worcester/Results"
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<h2> Curcumin Assays </h2> | <h2> Curcumin Assays </h2> | ||
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As can be seen, the EMG strain had the most pronounced biofilm development. While DH5α had an apparent drop in biofilm development, it like S. epidermidis and S. aureus showed an increase in biofilm growth. NCTC similarly shows an initial drop but an uptick in measured biofilm after 20 µM curcumin solution was applied. However, overlap between error bars in these four aforementioned strains among ratios means overall measurements may be suspect. | As can be seen, the EMG strain had the most pronounced biofilm development. While DH5α had an apparent drop in biofilm development, it like S. epidermidis and S. aureus showed an increase in biofilm growth. NCTC similarly shows an initial drop but an uptick in measured biofilm after 20 µM curcumin solution was applied. However, overlap between error bars in these four aforementioned strains among ratios means overall measurements may be suspect. | ||
Revision as of 17:32, 12 October 2018
Results
Biofilm Quantification
Antifreeze Proteins against Biofilm Formation
HIStag Purification
Protein Concentration Assay
SDS Page Gel
Biofilm Assay Result
Curcumin Assays
As can be seen, the EMG strain had the most pronounced biofilm development. While DH5α had an apparent drop in biofilm development, it like S. epidermidis and S. aureus showed an increase in biofilm growth. NCTC similarly shows an initial drop but an uptick in measured biofilm after 20 µM curcumin solution was applied. However, overlap between error bars in these four aforementioned strains among ratios means overall measurements may be suspect.
Lettuce Colony Counts
Gene Gun Transformation Results
Transformation with Antifreeze Proteins
Project Achievements
We achieved a lot over the course of our project, both positive and negative results. Below are our lists of successes and failures.
Successes of ICEberg
- Successful Purification of Antifreeze proteins in BL21 strains of E. coli
- Reduction of Biofilm formation with any application of
- Transformation of EMG cells to express Antifreeze protein
- Production of a functional and inexpensive Gene Gun
- Transformed lettuce leaves to express GFP using the team Gene Gun
Failures of ICEberg
- Inability to make competent NCTC cells with multiple protocols
- IPTG inducible operon did not turn on