Difference between revisions of "Team:WPI Worcester/Improve"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1> Improvement on 2015 WPI iGEM Team Project </h1>
  
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<h3>Antifreeze Proteins</h3>
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<p>The 2015 WPI iGEM Team attempted to analyze the biofilm inhibiting properties and antifreeze properties of various antifreeze proteins. The team was not able to prove that the E. coli was expressing the proteins being tests, so the results that were collected are not conclusively because of the proteins. This year, we decided to look further into the antimicrobial properties of these same antifreeze proteins. We were able to prove protein expression with a PAGE gel and use the bacteria expressing such proteins in various biofilm assays. From these assays, we could observe the effect of antifreeze proteins on the formation of biofilms. </p>
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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
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<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement. Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
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The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1809021">ZeAFP</a>
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<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1809035">IAFGP</a>
 
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Revision as of 21:58, 12 October 2018

Improvement on 2015 WPI iGEM Team Project

Antifreeze Proteins

The 2015 WPI iGEM Team attempted to analyze the biofilm inhibiting properties and antifreeze properties of various antifreeze proteins. The team was not able to prove that the E. coli was expressing the proteins being tests, so the results that were collected are not conclusively because of the proteins. This year, we decided to look further into the antimicrobial properties of these same antifreeze proteins. We were able to prove protein expression with a PAGE gel and use the bacteria expressing such proteins in various biofilm assays. From these assays, we could observe the effect of antifreeze proteins on the formation of biofilms.

ZeAFP IAFGP