Difference between revisions of "Team:CCU Taiwan/Safety"
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<li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a><hr> | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a><hr> | ||
<ul class="sub" id="sub_home" style="cursor:default;"> | <ul class="sub" id="sub_home" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home2">Medals</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home3">For Judges</li></a> | |
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<li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr> | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr> | ||
<ul class="sub" id="sub_project" style="cursor:default;"> | <ul class="sub" id="sub_project" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project6">Demonstration</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab "><li class="list" id="project5">InterLab</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr> | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr> | ||
<ul class="sub" id="sub_parts" style="cursor:default;"> | <ul class="sub" id="sub_parts" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts2">Basic Part</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts3">Composite Part</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Improve"><li class="list" id="parts4">Improved Part</li></a> | |
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</ul> | </ul> | ||
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<li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr> | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr> | ||
<ul class="sub" id="sub_modeling" style="cursor:default;"> | <ul class="sub" id="sub_modeling" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model4">Polymer</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Degradation"><li class="list" id="model3">Degradation</li></a> | |
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| − | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a> | + | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a><hr> |
<ul class="sub" id="sub_drylab" style="cursor:default;"> | <ul class="sub" id="sub_drylab" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr> | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr> | ||
<ul class="sub" id="sub_human_practice" style="cursor:default;"> | <ul class="sub" id="sub_human_practice" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Overview</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate"><li class="list" id="human_practice4">Intergrated HP</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a> | <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a> | ||
<ul class="sub" id="sub_notebook" style="cursor:default;"> | <ul class="sub" id="sub_notebook" style="cursor:default;"> | ||
| − | <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a> |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook2">Protocols</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook3">Experiments</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook4">Materials</li></a> | |
</ul> | </ul> | ||
</li> | </li> | ||
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</div> | </div> | ||
</nav> | </nav> | ||
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</header> | </header> | ||
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<div class="backgroundDrylab"> | <div class="backgroundDrylab"> | ||
<div class="bcg"></div> | <div class="bcg"></div> | ||
Revision as of 23:56, 13 October 2018
Safety
Genetically modified organisms escaping from lab is a serious problem since it will bring unpredictable impacts to our ecosystem. In our part design and production line, we were very conscious of biosafety. The yeast will pass the following process to achieve complete destruction. We also dealt cautiously with waste produced from the experiment and participated in safety training to ensure everyone conducting experiments had a minimal probability to cause biohazard pollution.
Filtering system
The average size of the P. pastoris used is about 4–6 μm [1], and the filter we used is 33 kDa, which is equivalent to the size of tens of nanometers [2], more than sufficient to trap all the yeast.
Heating system
According to the literature [3], heating at 70 °C for 30 minutes is sufficient to kill the cells. We used a temperature of 110 ° C for 10 minutes to kill any cells that might pass through the filter.
Reference
- 1. Gmeiner, C., Saadati, A., Maresch, D., Krasteva, S., Frank, M., Altmann, F., … Spadiut, O. (2015). Development of a fed-batch process for a recombinant Pichia pastoris Δoch1 strain expressing a plant peroxidase. Microbial Cell Factories, 14(1).
doi:10.1186/s12934-014-0183-3 - Bacher, G., Szymanski, W. W., Kaufman, S. L., Zöllner, P., Blaas, D., & Allmaier, G. (2001). Charge-reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses.
Journal of Mass Spectrometry, 36(9), 1038–1052.
doi:10.1002/jms.208 - 3. Martínez, D., Menéndez, C., Echemendia, F. M., Hernández, L., Sobrino, A., & Trujillo, L. E. (2015). Kinetics of sucrose hydrolysis by immobilized recombinant Pichia pastoris cells in a batch reactors. J Microb Biochem Technol, 7, 294-6.
- 4. Cregg, J. M., Barringer, K. J., Hessler, A. Y., & Madden, K. R. (1985). Pichia pastoris as a host system for transformations. Molecular and Cellular Biology, 5(12), 3376–3385.
doi:10.1128/mcb.5.12.3376 - 5. Ahmad, M., Hirz, M., Pichler, H., & Schwab, H. (2014). Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Applied Microbiology and Biotechnology, 98(12), 5301–5317.
doi:10.1007/s00253-014-5732-5