Difference between revisions of "Team:Nanjing-China/Design"
| Line 7: | Line 7: | ||
<link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Nanjing-China/CSS:loader?action=raw&ctype=text/css" /> | <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Nanjing-China/CSS:loader?action=raw&ctype=text/css" /> | ||
<style type="text/css"> | <style type="text/css"> | ||
| + | .button{ | ||
| + | width:60px; | ||
| + | height:40px; | ||
| + | line-height:40px; | ||
| + | z-index:5; | ||
| + | padding:2px; | ||
| + | alignment-adjust: central; | ||
| + | background-color:rgba(51,102,0,0.4); | ||
| + | border:rgba(153,153,153,1) 3px groove; | ||
| + | border-radius:8px; | ||
| + | } | ||
| + | .button:hover { | ||
| + | background-color:rgba(51,102,0,0.8); | ||
| + | cursor:pointer; | ||
| + | color:rgba(153,153,153,1); | ||
| + | border: 3px solid rgba rgba(0,153,0,0.6); | ||
| + | } | ||
</style> | </style> | ||
<script type="text/javascript" src="https://2018.igem.org/Team:Nanjing-China/Javascript:1?action=raw&ctype=text/javascript"></script> | <script type="text/javascript" src="https://2018.igem.org/Team:Nanjing-China/Javascript:1?action=raw&ctype=text/javascript"></script> | ||
Revision as of 08:41, 14 October 2018
Biosynthesis of CdS semiconductor
To construct our light-driven system, we first induce the precipitation of CdS semiconductor on the cell membrane. Two plasmids encoding the surface display protein OmpA-PbrR and the nitrogenase are co-transferred into E. coli strain. After Cd2+ is added into the media, the ions specifically bind to PbrR leading to aggregation of Cd2+ ions. At last when S2- ions are added into the media, E. coli cells form CdS semiconductor on the cell membrane because of the aggregation.
Light-driven nitrogen fixation in E. coli cells
To address the problem of electron transduction, CdS semiconductor act as semiconductors imitating the photosynthetic system under illumination. It provided excited electrons to a redox mediator methyl viologen (MV) which then penetrates into E. coli cells and transfer the electrons to Mo-Fe protein subunit of nitrogenase. Mo-Fe protein then utilizes the energy from these electrons to reduce dinitrogen to ammonia. The semiconductor regains its lost electron from sacrificial electron donors.
As a part of biohybrid system, the PbrR protein bears a high specificity. Our system is supposed to self-repair and can be built with a rather low cost. This design is of general applications as OmpA protein is only a surface display machinery for E. coli.
This part of the system is the expansion of our hydrogen production, and it proves that surface display machinery can be expanded to a general principle for biohybrid photosynthesis.
Device
To apply our system to the real world, we also designed a device consists of 3 modules: incubation module, illumination module and control module.
Address
Life Science Department
#163 Xianlin Blvd, Qixia District
Nanjing University
Nanjing, Jiangsu Province
P.R. of China
Zip: 210046
