Difference between revisions of "Team:CCU Taiwan/Composite Part"

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{{Template:CCU_Taiwan/css}}
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<meta name="viewport" content="width=device-width, initial-scale=1">
 
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$("#Parts").hover(function(){
 
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   <header>
 
   <header>
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         <nav class="navbar navbar-default navbar-inverse navbar-fixed-top">
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         <nav>
 
             <div class="container">
 
             <div class="container">
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                 <ul>
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                 <ul class="front">
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                     <div id="home_button" style="cursor:pointer;"  onclick="location.href=
 
                     <div id="home_button" style="cursor:pointer;"  onclick="location.href=
 
'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
 
'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
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                     <li class="title" id="Home"><a>Home</a>
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                         <ul class="sub" id="sub_home">
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                     <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a><hr>
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                            <li class="list" id="home1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Team">Team</a></li>
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                         <ul class="sub" id="sub_home" style="cursor:default;">
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<li class="list" id="home2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions">Attributions</a></li>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
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<li class="list" id="home3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Judge">For Judge</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home2">Medals</li></a>
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<div id="home_img">
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home3">For Judges</li></a>
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<img src="https://static.igem.org/mediawiki/2018/5/57/T--CCU_Taiwan--home.png"></img>
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</div>
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<div id="home_word">
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<p>There's a home for us, always.</p>
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</div>
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                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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                     <li class="title" id="Project"><a href="#">Project</a>
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                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr>
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<ul class="sub" id="sub_project">
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<ul class="sub" id="sub_project" style="cursor:default;">
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    <li class="list" id="project1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Description">Description</a></li>
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    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
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<li class="list" id="project2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Design">Design</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a>
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<li class="list" id="project3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Results">Results</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
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<li class="list" id="project4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements">Achievements</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
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<li class="list" id="project5"><a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab ">InterLab</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab "><li class="list" id="project5">InterLab</li></a>
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    <li class="list" id="project6"><a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate">Demonstration</a></li>
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</ul>
 
</ul>
 
</li>
 
</li>
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                     <li class="title" id="Parts"><a href="#">Parts</a>
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                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr>
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                        <ul class="sub" id="sub_parts">
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                        <ul class="sub" id="sub_parts" style="cursor:default;">
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    <li class="list" id="parts1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Parts">Parts</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
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<li class="list" id="parts2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part">Basic Part</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
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<li class="list" id="parts3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part">Composite Part</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Improve"><li class="list" id="parts3">Improved Part</li></a>
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<li class="list" id="parts4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Improve">Improved Part</a></li>
+
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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                     <li class="title" id="Modeling"><a href="#">Modeling</a>
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                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr>
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                         <ul class="sub" id="sub_modeling">
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                         <ul class="sub" id="sub_modeling" style="cursor:default;">
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                            <li class="list" id="model1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Model">Model</a></li>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
−
                            <li class="list" id="model2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Enzyme">Enzyme Activity</a></li>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding</li></a>
−
<li class="list" id="model3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Monolignol">Monolignol Binding</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer</li></a>
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<li class="list" id="model4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Tools">Tools</a></li>
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                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
                     <li class="title" id="Drylab"><a href="#">Drylab</a>
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                     <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a><hr>
−
                         <ul class="sub" id="sub_drylab">
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                         <ul class="sub" id="sub_drylab" style="cursor:default;">
−
                            <li class="list" id="drylab1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan">Our Plan</a></li>
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                            <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
−
<li class="list" id="drylab2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering">Engineering</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a>
−
<li class="list" id="drylab3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Safety">Safety</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
<li class="title" id="Human_Practice"><a href="#">Human Practice</a>
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    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr>
−
                         <ul class="sub" id="sub_human_practice">
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                         <ul class="sub" id="sub_human_practice" style="cursor:default;">
−
                             <li class="list" id="human_practice1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices">Human Practice</a></li>
+
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Overview</li></a>
−
<li class="list" id="human_practice2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Engagement">Engagement</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
−
<li class="list" id="human_practice3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship">Entrepreneurship</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
−
<li class="list" id="human_practice4"><a href="https://2018.igem.org/Team:CCU_Taiwan/Business_Model">Business Model</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate"><li class="list" id="human_practice5">Intergrated HP</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
−
<li class="title" id="Notebook"><a href="#">Notebook</a>
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    <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a>
−
                         <ul class="sub" id="sub_notebook">
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                         <ul class="sub" id="sub_notebook" style="cursor:default;">
−
                             <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook">Notebook</a></li>
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                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a>
−
<li class="list" id="notebook2"><a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design">Applied Design</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a>
−
<li class="list" id="notebook3"><a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments">Experiments</a></li>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook3">Protocols</li></a>
−
<li class="list" id="notebook4"><a href="https://2018.igem.org/Team:CCU_Taiwan/DATABASE">DATABASE</a></li>
+
<a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook4">Experiments</li></a>
 +
<a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook5">Materials</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
                     </li>
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         </nav>
 
         </nav>
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    </header>
 
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</body>
 
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<div class="column full_size judges-will-not-evaluate">
 
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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
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</div>
 
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<div class="clear"></div>
 
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<div class="column full_size">
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    </header>
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<h1>Composite Parts</h1>
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−
 
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<div class="backgroundParts">
−
 
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<div class="photoBasicPart"></div>
−
<p>
+
<div class="content">
−
A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
+
<p class="first">Composite Parts</p>
 +
<p class="second">BBa_K2809001 <br>
 +
Kozak sequence (Pichia pastoris)
 
</p>
 
</p>
 +
<p class="description">The Kozak sequence initiates the translation in Pichia pastoris. This Sequence is copied directly from pCore1 in reference .The start codon is not included.</p>
 +
 +
<p class="second">BBa_K2809002<br>
 +
Prepro-alpha-factor (codon modified for p.pastoris)
 +
</p>
 +
<p class="description">Prepro-alpha-factor is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs secreted expression of the recombinant protein, therefore, it allows the exportation of it. Once the recombinant protein is in the endoplasmic reticulum(ER) lumen, this secretion signal will be cleaved.</p>
 +
 +
<p class="second">BBa_K2809010<br>
 +
Mature Lac1 (laccase)
 +
</p>
 +
<p class="description">This is the codon-optimized version of laccase derived from Pinus taeda(Loblolly pine). Laccase is involved in monolignol dehydrogenation, using oxygen to oxidize their metal centers to enable catalytical phenol oxidation.It is able to produce dimers and oligomers of coniferyl alcohol, which is involved in the early stage of lignification.</p>
 +
<p class="description">The signal peptide have removed, so the sequence do NOT contain start codon. Furthermore, it includes HA-tag for Western Blot protein detection and 6xHis-tag for protein purification before stop codon.</p>
 +
 +
<p class="second">BBa_K2809020<br>
 +
Mature Px18 (peroxidase)
 +
</p>
 +
<p class="second">BBa_K2809030<br>
 +
Mature Px16 (peroxidase)
 +
</p>
 +
<p class="description">Those are the codon-optimized version of peroxidase derived from Picea abies(Norway spruce). Peroxidases (Px16 and Px18) are involved in monolignol dehydrogenation, using hydrogen peroxide as a substrate. They are able to form polymers from oligomers or dimers, which contribute to lignin polymerization.</p>
 +
<p class="description">The signal peptide have removed, so the sequence do NOT contain start codon.Moreover, it includes V5-tag for Western Blot protein detection and  6xHis-tag for protein purification before stop codon.</p>
 +
<p class="description">The signal peptide have removed, so the sequence do NOT contain start codon.Furthermore, it includes FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification before stop codon.</p>
 +
<p class="description"><br>Best New Composite parts: </p>
 +
<p class="second">BBa_K2809011<br>
 +
laccase 1
 +
</p>
 +
<p class="description">We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809010.<br>
 +
  This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of laccase gene sequence, HA tag for Western Blot protein detection and 6xHis-tag for protein purification.
 +
</p>
 +
<p class="second">BBa_K2809021<br>
 +
peroxidase 18
 +
</p>
 +
<p class="description">  We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809020.<br>
 +
  This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 18 gene sequence, V5-tag for Western Blot protein detection and 6xHis-tag for protein purification.
 +
 +
</p>
 +
<p class="second">BBa_K2809031<br>
 +
peroxidase 16
 +
</p>
 +
<p class="description">  We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809030.<br>
 +
  This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 16 gene sequence, FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification.
  
−
<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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</p>
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</div>
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</div>
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</div>
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<div class="column full_size">
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</body>
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<div class="highlight decoration_background">
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<footer class="footer">
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<h3>Note</h3>
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            <a href="http://huitong.com.tw">
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Revision as of 10:46, 14 October 2018

Composite Parts

BBa_K2809001
Kozak sequence (Pichia pastoris)

The Kozak sequence initiates the translation in Pichia pastoris. This Sequence is copied directly from pCore1 in reference .The start codon is not included.

BBa_K2809002
Prepro-alpha-factor (codon modified for p.pastoris)

Prepro-alpha-factor is the secretion signal peptide from the yeast α-mating factor. This signal peptide directs secreted expression of the recombinant protein, therefore, it allows the exportation of it. Once the recombinant protein is in the endoplasmic reticulum(ER) lumen, this secretion signal will be cleaved.

BBa_K2809010
Mature Lac1 (laccase)

This is the codon-optimized version of laccase derived from Pinus taeda(Loblolly pine). Laccase is involved in monolignol dehydrogenation, using oxygen to oxidize their metal centers to enable catalytical phenol oxidation.It is able to produce dimers and oligomers of coniferyl alcohol, which is involved in the early stage of lignification.

The signal peptide have removed, so the sequence do NOT contain start codon. Furthermore, it includes HA-tag for Western Blot protein detection and 6xHis-tag for protein purification before stop codon.

BBa_K2809020
Mature Px18 (peroxidase)

BBa_K2809030
Mature Px16 (peroxidase)

Those are the codon-optimized version of peroxidase derived from Picea abies(Norway spruce). Peroxidases (Px16 and Px18) are involved in monolignol dehydrogenation, using hydrogen peroxide as a substrate. They are able to form polymers from oligomers or dimers, which contribute to lignin polymerization.

The signal peptide have removed, so the sequence do NOT contain start codon.Moreover, it includes V5-tag for Western Blot protein detection and 6xHis-tag for protein purification before stop codon.

The signal peptide have removed, so the sequence do NOT contain start codon.Furthermore, it includes FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification before stop codon.


Best New Composite parts:

BBa_K2809011
laccase 1

We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809010.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of laccase gene sequence, HA tag for Western Blot protein detection and 6xHis-tag for protein purification.

BBa_K2809021
peroxidase 18

We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809020.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 18 gene sequence, V5-tag for Western Blot protein detection and 6xHis-tag for protein purification.

BBa_K2809031
peroxidase 16

We use pGAPZ A vector from invitrogen and insert this part to construct our vector. We constructed this part by combining BBa_K2809001, BBa_K2809002 and BBa_K2809030.
This part contain a Kozak sequence for initiating and enhancing translation, Prepro-alpha-factor as a signal peptide for secretion, codon-optimized version of peroxidase 16 gene sequence, FLAG-tag for Western Blot protein detection and 6xHis-tag for protein purification.